Abstract：
Aeromonas hydrophila, a Gram-negative zoonotic bacterium, causes high mortality in fish farming and immunocompromised patients. This study aimed to extract methyl gallate (MG) from the flowers of Camellia nitidissima Chi and evaluate its potential as a quorum sensing inhibitor (QSI) against Aeromonas hydrophila SHAe 115. MG reduced QS-associated virulence factors, including hemolysis, protease, and lipase, while impairing swimming motility and biofilm formation. Additionally, MG down-regulated positive regulatory genes (ahyR, fleQ) and up-regulated negative regulators (litR, fleN). This highlights MG\'s promise as a potent QSI for A. hydrophila SHAe 115, advancing strategies against infections in aquaculture and human health.
摘要：
嗜水气单胞菌,革兰氏阴性人畜共患病细菌，导致鱼类养殖和免疫功能低下患者的高死亡率。本研究旨在从山茶花中提取没食子酸甲酯（MG），并评估其作为针对嗜水气单胞菌SHAe115的群体感应抑制剂（QSI）的潜力。MG减少QS相关毒力因子，包括溶血,蛋白酶，还有脂肪酶,同时损害游泳运动和生物膜形成。此外,MG下调正调节基因(ahyR，fleQ)和上调的负调节剂(litR，fleN).这凸显了MG作为A.hydrophilaSHAe115的有效QSI的承诺，推进了水产养殖和人类健康中针对感染的策略。
