RESULTS: Eighty nine (89) Stemphylium isolates were obtained from naturally infected tomatoes as well as S. paniculatum, potato, eggplant, scarlet eggplant (Solanum aethiopicum var. gilo), Physalis angulata, and Capsicum species. Phylogenetic analyses encompassing the ITS-5.8S rDNA and glyceraldehyde-3-phosphate dehydrogenase genomic regions placed the isolates into two distinct groupings with either Stemphylium lycopersici or S. solani. Isolates of S. lycopersici (n = 81) were obtained infecting tomato, potato, eggplant, S. paniculatum, and P. angulata. Isolates of S. solani (n = 8) were detected in natural association with scarlet eggplant and tomato. Two isolates of S. lycopersici displayed a wide experimental host range in greenhouse bioassays, infecting accessions of 12 out of 18 species. Ocimum basilicum (Lamiaceae) was the only experimental host outside the Solanaceae family.
结果:从自然感染的番茄以及S.paniculatum中获得了八十九(89)个Stemphylium分离株,马铃薯,茄子,猩红色茄子(S.aethiopicumvar.gilo),酸浆,和辣椒物种。包含ITS-5.8SrDNA和甘油醛-3-磷酸脱氢酶基因组区域的系统发育分析将分离株分为两个不同的组,分别是S.lycopersici或S.solani。获得了感染番茄的番茄分离株(n=81),马铃薯,茄子,S.paniculatum,还有P.angulata.检测到solaniS.solani(n=8)与猩红色茄子和番茄自然结合。在温室生物测定中,两种分离的lycopersici表现出广泛的实验宿主范围,感染18种中的12种。罗勒(Lamiaceae)是茄科以外的唯一实验宿主。
■白斑病发病率较高,更广泛的主机范围,它是茄科疾病中传播更多的病原体,在巴西所有宏观区域都被检测到。因此,这种病原体应该是旨在开发抗性茄科品种的育种计划的重点。