PDF(Pubmed)
Abstract：
Pollution and its effects have been of major concern in recent decades. Many strategies and markers have been developed to assess their effects on biota. Cytochrome P450 (CYP) genes have received significant attention in this context because of their relationship with detoxification and activation of exogenous compounds. While their expression has been identified as a pollution exposure biomarker, in most cases, it has been tested only after acute exposures and for CYP genes associated with exogenous compounds. To elucidate CYP gene expression patterns under chronic pollution exposure, we have used the silverside Basilichthys microlepidotus as a model, which inhabits the Maipo River Basin, a freshwater system with different pollution levels. We performed next-generation RNA sequencing of liver and gill tissues from polluted and non-polluted populations. We found most CYP genes were not dysregulated by pollution, and the seven genes that were present and differentially expressed in liver and gill were mainly downregulated. Three CYP genes associated with exogenous compounds showed differential expression in the gill, while four CYP genes associated with endogenous compounds showed differential expression in the liver. The findings presented here highlight the importance of CYP genes, his family, tissues and his interaction in the context of pollution biomarkers use.
摘要：
近几十年来，污染及其影响一直是人们关注的主要问题。已经开发了许多策略和标记来评估它们对生物群的影响。细胞色素P450(CYP)基因由于其与外源化合物的解毒和活化的关系而在这方面受到了极大的关注。虽然它们的表达已被确定为污染暴露生物标志物，在大多数情况下,仅在急性暴露后以及与外源化合物相关的CYP基因进行了测试。为了阐明慢性污染暴露下CYP基因的表达模式，我们已经使用了银边Basilichthysmicrolepidotus作为模型，栖息在麦坡河流域，不同污染程度的淡水系统。我们对污染和未污染人群的肝脏和g组织进行了下一代RNA测序。我们发现大多数CYP基因没有因污染而失调，在肝脏和g中存在和差异表达的七个基因主要被下调。与外源化合物相关的三个CYP基因在the中显示出差异表达，而与内源性化合物相关的四个CYP基因在肝脏中显示出差异表达。这里的发现强调了CYP基因的重要性，他的家人,组织和他的相互作用在污染生物标志物的使用。
