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Abstract：
UNASSIGNED: This study aims to evaluate the effect of Clostridium perfringens sialidase treatment on monolayer cell behavior using computational screening and an in vitro approach to demonstrate interaction between enzyme-based drugs and ligands in host cells.
UNASSIGNED: The in silico study was carried out by molecular docking analysis used to predict the interactions between atoms that occur, followed by genetic characterization of sialidase from a wild isolate. Sialidase, which has undergone further production and purification processes exposed to chicken embryonic fibroblast cell culture, and observations-based structural morphology of cells compared between treated cells and normal cells without treatment.
UNASSIGNED: Based on an in silico study, C. perfringens sialidase has an excellent binding affinity with Neu5Acα (2.3) Gal ligand receptor with Gibbs energy value (∆G)-7.35 kcal/mol and Ki value of 4.11 µM. Wild C. perfringens isolates in this study have 99.1%-100% similarity to the plc gene, NanH, and NanI genes, while NanJ shows 93.18% similarity compared to the reference isolate from GenBank. Sialidase at 750 and 150 mU may impact the viability, cell count, and cell behavior structure of fibroblast cells by significantly increasing the empty area and perimeter of chicken embryo fibroblast (CEF) cells, while at 30 mU sialidase shows no significant difference compared with mock control.
UNASSIGNED: Sialidase-derived C. perfringens has the capacity to compete with viral molecules for attachment to host sialic acid based on in silico analysis. However, sialidase treatment has an impact on monolayer cell fibroblasts given exposure to high doses.
UNASSIGNED: The in silico study was carried out by molecular docking analysis used to predict the interactions between atoms that occur, followed by genetic characterization of sialidase from a wild isolate. Sialidase, which has undergone further production and purification processes exposed to chicken embryonic fibroblast cell culture, and observations-based structural morphology of cells compared between treated cells and normal cells without treatment.
UNASSIGNED: Based on an in silico study, C. perfringens sialidase has an excellent binding affinity with Neu5Acα (2.3) Gal ligand receptor with Gibbs energy value (∆G)-7.35 kcal/mol and Ki value of 4.11 µM. Wild C. perfringens isolates in this study have 99.1%-100% similarity to the plc gene, NanH, and NanI genes, while NanJ shows 93.18% similarity compared to the reference isolate from GenBank. Sialidase at 750 and 150 mU may impact the viability, cell count, and cell behavior structure of fibroblast cells by significantly increasing the empty area and perimeter of chicken embryo fibroblast (CEF) cells, while at 30 mU sialidase shows no significant difference compared with mock control.
UNASSIGNED: Sialidase-derived C. perfringens has the capacity to compete with viral molecules for attachment to host sialic acid based on in silico analysis. However, sialidase treatment has an impact on monolayer cell fibroblasts given exposure to high doses.
摘要：
■本研究旨在使用计算筛选和体外方法来评估产气荚膜梭菌唾液酸酶处理对单层细胞行为的影响，以证明基于酶的药物与宿主细胞中配体之间的相互作用。
■计算机模拟研究是通过分子对接分析进行的，用于预测发生的原子之间的相互作用，随后是来自野生分离物的唾液酸酶的遗传表征。唾液酸酶,它经历了进一步的生产和纯化过程，暴露于鸡胚胎成纤维细胞培养，和基于观察的细胞结构形态比较处理的细胞和未处理的正常细胞。
■基于计算机模拟研究，产气荚膜梭菌唾液酸酶与Neu5Acα（2.3）Gal配体受体具有优异的结合亲和力，吉布斯能量值（ΔG）-7.35kcal/mol和Ki值为4.11µM。本研究中野生产气荚膜梭菌分离株与plc基因有99.1%-100%的相似性，NanH,和NanI基因，而NanJ与来自GenBank的参考分离株相比显示93.18%的相似性。750和150mU的唾液酸酶可能会影响生存力，细胞计数,显著增加鸡胚成纤维细胞(CEF)的空区和周长,而在30mU唾液酸酶与模拟对照相比没有显着差异。
■基于计算机模拟分析,源自唾液酸酶的产气荚膜梭菌具有与病毒分子竞争附着宿主唾液酸的能力。然而,唾液酸酶处理对暴露于高剂量的单层细胞成纤维细胞有影响。
■计算机模拟研究是通过分子对接分析进行的，用于预测发生的原子之间的相互作用，随后是来自野生分离物的唾液酸酶的遗传表征。唾液酸酶,它经历了进一步的生产和纯化过程，暴露于鸡胚胎成纤维细胞培养，和基于观察的细胞结构形态比较处理的细胞和未处理的正常细胞。
■基于计算机模拟研究，产气荚膜梭菌唾液酸酶与Neu5Acα（2.3）Gal配体受体具有优异的结合亲和力，吉布斯能量值（ΔG）-7.35kcal/mol和Ki值为4.11µM。本研究中野生产气荚膜梭菌分离株与plc基因有99.1%-100%的相似性，NanH,和NanI基因，而NanJ与来自GenBank的参考分离株相比显示93.18%的相似性。750和150mU的唾液酸酶可能会影响生存力，细胞计数,显著增加鸡胚成纤维细胞(CEF)的空区和周长,而在30mU唾液酸酶与模拟对照相比没有显着差异。
■基于计算机模拟分析,源自唾液酸酶的产气荚膜梭菌具有与病毒分子竞争附着宿主唾液酸的能力。然而,唾液酸酶处理对暴露于高剂量的单层细胞成纤维细胞有影响。
