Abstract：
Microorganisms, such as yeasts, filamentous fungi, bacteria, and microalgae, have gained significant attention due to their potential in producing commercially valuable natural carotenoids. In recent years, Phaffia rhodozyma yeasts have emerged as intriguing non-conventional sources of carotenoids, particularly astaxanthin and β-carotene. However, the shift from academic exploration to effective industrial implementation has been challenging to achieve. This study aims to bridge this gap by assessing various scenarios for carotenoid production and recovery. It explores the use of ionic liquids (ILs) and bio-based solvents (ethanol) to ensure safe extraction. The evaluation includes a comprehensive analysis involving Life Cycle Assessment (LCA), biocompatibility assessment, and Techno-Economic Analysis (TEA) of two integrated technologies that utilize choline-based ILs and ethanol (EtOH) for astaxanthin (+β-carotene) recovery from P. rhodozyma cells. This work evaluates the potential sustainability of integrating these alternative solvents within a yeast-based bioeconomy.
摘要：
微生物,如酵母，丝状真菌,细菌,和微藻，由于它们在生产有商业价值的天然类胡萝卜素方面的潜力而获得了极大的关注。近年来,红法夫酵母已经成为吸引人的非常规类胡萝卜素来源，特别是虾青素和β-胡萝卜素。然而,从学术探索到有效的工业实施的转变一直具有挑战性。这项研究旨在通过评估类胡萝卜素生产和回收的各种情况来弥合这一差距。它探讨了离子液体（IL）和生物基溶剂（乙醇）的使用，以确保安全提取。评估包括涉及生命周期评估(LCA)的综合分析，生物相容性评估，和技术经济分析（TEA）的两种集成技术，这些技术利用基于胆碱的IL和乙醇（EtOH）从P.rhodozyma细胞中回收虾青素（β-胡萝卜素）。这项工作评估了在基于酵母的生物经济中整合这些替代溶剂的潜在可持续性。
