Abstract：
Obesity is a persistent metabolic condition resulting from the excessive accumulation or abnormal distribution of body fat. This study aimed to establish an experimental rat model of obesity. The efficacy of treating obesity with Hedan tablets (HDT) was assessed by monitoring changes in weight, blood lipid levels, analyzing inflammatory factors, evaluating organ indices, and observing liver tissue pathology. Furthermore, we utilized 16S ribosomal RNA gene sequencing technology to explore changes in intestinal flora. In addition, GC-MS was used to measure fecal short-chain fatty acid (SCFA) content. The onset of obesity led to a significant decrease in the relative abundance of beneficial bacteria. Conversely, the administration of HDT demonstrated a substantial ability to increase the relative abundance of beneficial bacteria. Obesity resulted in a noteworthy reduction in total SCFAs, a trend significantly reversed in the HDT group. Through correlation analysis, it was determined that HDT mitigated the inflammatory response and improved blood lipid levels by augmenting the abundance of Lactobacillus, Limosilactobacillus, Ruminococcus, and Enterococcus. These particular intestinal flora were identified as regulators of SCFA metabolism, thereby ameliorating metabolic abnormalities associated with obesity. Moreover, HDT intervention elevated the overall fecal concentration of SCFAs, thereby improving metabolic disorders induced by obesity. The anti-obesity effects of HDT are likely attributable to their capacity to influence the composition of intestinal flora and boost SCFA levels in the intestine.
摘要：
肥胖是由体内脂肪的过度积累或异常分布导致的持续代谢状况。本研究旨在建立实验性肥胖大鼠模型。通过监测体重变化来评估用荷丹片（HDT）治疗肥胖症的功效，血脂水平，分析炎症因子，评估器官指数，并观察肝脏组织病理。此外，我们利用16S核糖体RNA基因测序技术探索肠道菌群的变化。此外,使用GC-MS测量粪便短链脂肪酸(SCFA)含量。肥胖的发作导致有益细菌的相对丰度显著降低。相反，HDT的给药显示出显著增加有益细菌相对丰度的能力。肥胖导致总SCFA显著减少，HDT组的趋势显着逆转。通过相关性分析,确定HDT通过增加乳酸菌的丰度来减轻炎症反应并改善血脂水平，Limosilactacillus,Ruminococus,和肠球菌.这些特定的肠道菌群被确定为SCFA代谢的调节剂，从而改善与肥胖相关的代谢异常。此外，HDT干预提高了SCFA的总粪便浓度，从而改善肥胖引起的代谢紊乱。HDT的抗肥胖作用可能归因于它们影响肠道菌群组成和提高肠道中SCFA水平的能力。
