Abstract：
Eimeria tenella is the most pathogenic and harmful intestinal parasitic protozoan. Recombinant DNA vaccines open options for promising strategies for preventing avian coccidiosis, replacing chemical drugs and live oocyst vaccines. Two important antigenic proteins, EtAMA3 (also known as SporoAMA1) and EtRON2L2, act together to promote the invasion of E. tenella sporozoites. In this study, a recombinant DNA vaccine, designated pcDNA3.1(+)-AR, was constructed based on EtAMA3DII, EtRON2L2D3, and EtRON2L2D4. Chickens were intramuscularly immunized with different doses (25, 50, or 100 μg) of pcDNA3.1(+)-AR to evaluate its immunoprotective effects in vivo. The chickens in the 50 μg and 100 μg groups had higher cytokine concentrations (interleukin 2, interferon-gamma, and interleukin 10), and lesion scores (81.9% and 67.57%, respectively) and relative oocyst production (47% and 19%, respectively) reduced compared with the unchallenged group, indicating partial protection against E. tenella. These results suggest that pcDNA3.1(+)-AR is a promising vaccine candidate against avian coccidiosis.
摘要：
柔嫩艾美耳球虫是最致病和有害的肠道寄生原虫。重组DNA疫苗为预防禽球虫病的有希望的策略打开了选择，替代化学药物和活卵囊疫苗。两种重要的抗原蛋白，EtAMA3（也称为SporoAMA1）和EtRON2L2共同作用以促进E.tenella子孢子的入侵。在这项研究中,重组DNA疫苗,指定pcDNA3.1(+)-AR,是基于EtAMA3DII建造的，EtRON2L2D3和EtRON2L2D4。用不同剂量（25、50或100μg）的pcDNA3.1（）-AR对鸡进行肌内免疫，以评估其体内免疫保护作用。50μg和100μg组中的鸡具有较高的细胞因子浓度（白介素2，干扰素-γ，和白细胞介素10），和病变评分（81.9%和67.57%，分别)和相对卵囊产量(47%和19%，分别)与无挑战组相比减少，表明对E.tenella的部分保护。这些结果表明，pcDNA3.1（）-AR是一种有希望的针对禽球虫病的疫苗候选物。
