Abstract：
Ticks are hematophagous arthropods and, during feeding, may transmit pathogens to vertebrate hosts, including humans. This study aimed to investigate the presence of Rickettsia spp. in ticks collected between 2010 and 2013 from free-ranging capybaras (Hydrochoerus hydrochaeris) and opossums (Didelphis albiventris) that inhabit Sabiá Park in Uberlândia, Brazil. Overall, 1,860 ticks were collected: 1,272 (68.4%) from capybaras (487 of the species Amblyomma sculptum, 475 adults and 12 nymphs; 778 Amblyomma dubitatum, 727 adults and 51 nymphs; and seven larva clusters of the genus Amblyomma); and 588 (31.6%) from opossums (21 A. sculptum, one adult and 20 nymphs; 79 A. dubitatum, all nymphs; 15 Ixodes loricatus, 12 adults and three nymphs; 457 Amblyomma sp. larva clusters; 15 Ixodes sp. larva clusters; and one Argasidae larva cluster). Out of 201 DNA samples tested for the presence of Rickettsia spp. DNA using polymerase chain reaction (PCR) 12 showed amplification of a gtlA gene segment that was specific to Rickettsia bellii, a bacterium non-pathogenic to humans. As there has been a report showing serological evidence of infections caused by Rickettsia species of the spotted fever group (SFG) in capybaras and opossums in the park, including Rickettsia rickettsii, the etiological agent of Brazilian spotted fever, and considering the presence of A. sculptum ticks, which are aggressive to humans, as well as these vertebrate hosts, which are amplifiers of R. rickettsii, it is important to monitor the presence of SFG rickettsiae in the Sabiá Park, which is visited daily by thousands of people.
摘要：
蜱是吸血节肢动物，在喂养过程中，可能会将病原体传播给脊椎动物宿主，包括人类。本研究旨在调查立克次体的存在。在2010年至2013年之间从居住在乌伯拉迪亚Sabiá公园中的自由放养的capybaras（Hydrochoerushydrochaeris）和负鼠（Didelphisalbiventris）中收集的蜱虫，巴西。总的来说,收集了1,860只蜱虫：1,272只（68.4％）来自Capybaras（487种Amblyomma雕塑，475个成人和12个若虫；778个弱小的dubitatum，727只成虫和51只若虫；以及七个弱虫属的幼虫簇）；和588（31.6％）来自负鼠（21A。雕塑，一个成年和20个若虫；79A.dubitatum，所有若虫；15只若虫，12个成虫和3个若虫；457个弱虫。幼虫簇；15个Ixodessp.幼虫簇；和一个Argasidae幼虫簇）。在201个DNA样品中，测试了立克次体的存在。使用聚合酶链反应（PCR）12的DNA显示了对立克次体bellii特异的gtlA基因片段的扩增，对人类无致病性的细菌。由于有报告显示血清学证据表明，在公园中的水蛭和负鼠中，斑点热组（SFG）的立克次体引起的感染，包括立克次体立克次体，巴西斑点热的病原体，考虑到A.雕塑蜱的存在，对人类具有攻击性，以及这些脊椎动物宿主，这是R.Rickettsii的放大器，重要的是要监测萨比阿公园中SFG立克次体的存在，每天都有成千上万的人参观。
