PDF(Pubmed)
Abstract：
One-sided vestibular disorders are common in clinical practice; however, their models have not been fully established. We investigated the effect of unilateral or bilateral deficits in the vestibular organs on the vestibulo-ocular reflex (VOR) and optokinetic reflex (OKR) of zebrafish using in-house equipment. For physical dislodgement of the otoliths in the utricles of zebrafish larvae, one or both utricles were separated from the surrounding tissue using glass capillaries. The video data from VOR and OKR tests with the larvae was collected and processed using digital signal processing techniques such as fast Fourier transform and low-pass filters. The results showed that unilateral and bilateral damage to the vestibular system significantly reduced VOR and OKR. In contrast, no significant difference was observed between unilateral and bilateral damage. This study confirmed that VOR and OKR were significantly reduced in zebrafish with unilateral and bilateral vestibular damage. Follow-up studies on unilateral vestibular disorders can be conducted using this tool.
摘要：
单侧前庭疾病在临床实践中很常见；然而，他们的模式还没有完全建立起来。我们使用内部设备研究了前庭器官单侧或双侧缺陷对斑马鱼前庭眼反射（VOR）和视动反射（OKR）的影响。对于斑马鱼幼虫中耳石的物理移位，使用玻璃毛细血管将一个或两个细胞与周围组织分离。使用快速傅里叶变换和低通滤波器等数字信号处理技术收集和处理来自幼虫的VOR和OKR测试的视频数据。结果表明，单侧和双侧前庭系统损伤显著降低VOR和OKR。相比之下,单侧和双侧损伤之间无显著差异.本研讨证实斑马鱼单侧和双侧前庭毁伤的VOR和OKR均显著降低。可以使用该工具进行单侧前庭疾病的后续研究。
