PDF(Pubmed)
Abstract：
A positive Mycoplasma hyopneumoniae PCR result in a clinical specimen may eventually represent the mere detection of non-viable bacteria, complicating the diagnostic interpretation. Thus, the objective of this study was to evaluate the PCR detection of non-viable M. hyopneumoniae and its residual cell-free DNA in live pigs. Pigs were inoculated with either active or inactivated M. hyopneumoniae and were sampled for up to 14 days. Mycoplasma hyopneumoniae was not detected by PCR at any timepoint in pigs inoculated with the inactivated bacterium, suggesting that in healthy pigs, the non-viable M. hyopneumoniae DNA was rapidly sensed and cleared.
摘要：
临床标本中猪肺炎支原体PCR阳性结果可能最终代表仅检测到无活力细菌，复杂的诊断解释。因此，本研究的目的是评估PCR检测活猪非活猪肺炎支原体及其残留的无细胞DNA。用活性或灭活的猪肺炎支原体接种猪,取样长达14天。在接种灭活细菌的猪中，在任何时间点通过PCR均未检测到猪肺炎支原体，这表明在健康的猪中，无法存活的猪肺炎支原体DNA被快速检测和清除。
