PDF(Pubmed)
Abstract：
Iron is an essential element that plays critical roles in many biological/metabolic processes, ranging from oxygen transport, mitochondrial respiration, to host defense and cell signaling. Maintaining an appropriate iron level in the body is vital to the human health. Iron deficiency or overload can cause life-threatening conditions. Thus, developing a new, rapid, cost-effective, and easy to use method for iron detection is significant not only for environmental monitoring but also for disease prevention. In this study, we report an innovative Fe3+ detection strategy by using both a ligand probe and an engineered nanopore with two binding sites. In our design, one binding site of the nanopore has a strong interaction with the ligand probe, while the other is more selective toward interfering species. Based on the difference in the number of ligand DTPMPA events in the absence and presence of ferric ions, micromolar concentrations of Fe3+ could be detected within minutes. Our method is selective: micromolar concentrations of Mg2+, Ca2+, Cd2+, Zn2+, Ni2+, Co2+, Mn2+, and Cu2+ would not interfere with the detection of ferric ions. Furthermore, Cu2+, Ni2+, Co2+, Zn2+, and Mn2+ produced current blockage events with quite different signatures from each other, enabling their simultaneous detection. In addition, simulated water and serum samples were successfully analyzed. The nanopore sensing strategy developed in this work should find useful application in the development of stochastic sensors for other substances, especially in situations where multi-analyte concurrent detection is desired.
摘要：
铁是一种在许多生物/代谢过程中起关键作用的必需元素。从氧气运输，线粒体呼吸，宿主防御和细胞信号。保持体内适当的铁水平对人体健康至关重要。铁缺乏或超负荷可导致危及生命的状况。因此，开发新的,快速,成本效益高,并且易于使用的铁检测方法不仅对环境监测而且对疾病预防具有重要意义。在这项研究中,我们报告了通过使用配体探针和具有两个结合位点的工程化纳米孔的创新Fe3+检测策略。在我们的设计中，纳米孔的一个结合位点与配体探针有很强的相互作用，而另一种对干扰物种更具选择性。基于在不存在和存在三价铁离子的情况下配体DTPMPA事件的数量差异，可以在几分钟内检测到微摩尔浓度的Fe3+。我们的方法是选择性的：Mg2+的微摩尔浓度，Ca2+,Cd2+,Zn2+,Ni2+,Co2+,Mn2+,和Cu2+不会干扰三价铁离子的检测。此外，Cu2+,Ni2+,Co2+,Zn2+,和Mn2+产生了电流阻塞事件，它们具有彼此完全不同的特征，使其同时检测。此外,成功分析了模拟水和血清样品。在这项工作中开发的纳米孔传感策略应该在其他物质的随机传感器的开发中找到有用的应用。特别是在需要多分析物同时检测的情况下。
