Abstract：
OBJECTIVE: Periodontitis is an inflammatory disease that destroys periodontal tissues. Interleukin-20 (IL-20), on the other hand, is known as a potent angiogenic, chemotactic, and pro-inflammatory cytokine associated with various chronic inflammatory disorders. IL-20 has a significant role in the regulation of osteoclastogenesis and osteoblastogenesis. This study aimed to evaluate the effect of IL-20 on periodontal destruction.
METHODS: In this study, a total of 60 participants were included, 30 of whom were systemically and periodontally healthy (control group), and 30 were systemically healthy but had periodontitis (periodontitis group). Gingival crevicular fluid (GCF) and serum samples were collected from the participants for biochemical analysis. Enzyme-linked immunosorbent assay was used to determine the levels of IL-20, tumor necrosis factor (TNF)-α, IL1β/IL-10, RANKL/osteoprotegerin (OPG), and matrix metalloproteinase-8 (MMP8). For statistical analysis, the independent t-test, Pearson correlation coefficients, and the Chi-square test were used.
RESULTS: GCF IL-20, RANKL, RANKL/OPG, serum IL-20, RANKL, RANKL/OPG, MMP-8, TNF-α, IL-1B, and IL-1β/IL-10 values were found to be statistically significantly higher in the periodontitis group than in the control group. GCF OPG and serum IL-10 values were found to be statistically significantly higher in the control group than in the periodontitis group. No statistically significant difference was observed between the groups in serum OPG values. A statistically significantly positive correlation was observed between serum IL-20 value and serum RANKL, RANKL/OPG, MMP-8, TNF-α, IL-1β values, and periodontal clinical parameters. The ROC curves showed: AUC = 0.788 for GCF IL-20, and AUC = 1.000 for serum IL-20.
CONCLUSIONS: According to the results of the study, IL-20 was found to be associated with periodontitis. The role of IL-20 in periodontal pathogenesis is related to osteoclastogenesis and collagen degradation. It is conceivable that IL-20 may increase bone destruction by both affecting the RANKL/OPG ratio and proinflammatory cytokines.
METHODS: In this study, a total of 60 participants were included, 30 of whom were systemically and periodontally healthy (control group), and 30 were systemically healthy but had periodontitis (periodontitis group). Gingival crevicular fluid (GCF) and serum samples were collected from the participants for biochemical analysis. Enzyme-linked immunosorbent assay was used to determine the levels of IL-20, tumor necrosis factor (TNF)-α, IL1β/IL-10, RANKL/osteoprotegerin (OPG), and matrix metalloproteinase-8 (MMP8). For statistical analysis, the independent t-test, Pearson correlation coefficients, and the Chi-square test were used.
RESULTS: GCF IL-20, RANKL, RANKL/OPG, serum IL-20, RANKL, RANKL/OPG, MMP-8, TNF-α, IL-1B, and IL-1β/IL-10 values were found to be statistically significantly higher in the periodontitis group than in the control group. GCF OPG and serum IL-10 values were found to be statistically significantly higher in the control group than in the periodontitis group. No statistically significant difference was observed between the groups in serum OPG values. A statistically significantly positive correlation was observed between serum IL-20 value and serum RANKL, RANKL/OPG, MMP-8, TNF-α, IL-1β values, and periodontal clinical parameters. The ROC curves showed: AUC = 0.788 for GCF IL-20, and AUC = 1.000 for serum IL-20.
CONCLUSIONS: According to the results of the study, IL-20 was found to be associated with periodontitis. The role of IL-20 in periodontal pathogenesis is related to osteoclastogenesis and collagen degradation. It is conceivable that IL-20 may increase bone destruction by both affecting the RANKL/OPG ratio and proinflammatory cytokines.
摘要：
目的：牙周炎是一种破坏牙周组织的炎症性疾病。白细胞介素-20(IL-20),另一方面,被称为有效的血管生成，趋化,和与各种慢性炎症相关的促炎细胞因子。IL-20在破骨细胞生成和成骨细胞生成的调节中具有重要作用。本研究旨在评价IL-20对牙周破坏的影响。
方法：在本研究中，共包括60名参与者，其中30例全身和牙周健康（对照组），30例全身健康但有牙周炎(牙周炎组)。从参与者收集牙龈沟液（GCF）和血清样品进行生化分析。采用酶联免疫吸附试验检测IL-20、肿瘤坏死因子(TNF)-α、IL1β/IL-10，RANKL/骨保护素（OPG），和基质金属蛋白酶-8(MMP8)。为了进行统计分析，独立t检验，皮尔逊相关系数，并采用卡方检验。
结果：GCFIL-20，RANKL，RANKL/OPG,血清IL-20，RANKL，RANKL/OPG,MMP-8,TNF-α,IL-1B,发现牙周炎组的IL-1β/IL-10值在统计学上明显高于对照组。发现对照组的GCFOPG和血清IL-10值在统计学上明显高于牙周炎组。两组之间的血清OPG值没有观察到统计学上的显着差异。血清IL-20值与血清RANKL呈显著正相关,RANKL/OPG,MMP-8,TNF-α,IL-1β值，和牙周临床参数。ROC曲线显示:GCFIL-20的AUC=0.788,血清IL-20的AUC=1.000。
结论：根据研究结果，发现IL-20与牙周炎有关。IL-20在牙周发病中的作用与破骨细胞生成和胶原降解有关。可以想象,IL-20可以通过影响RANKL/OPG比率和促炎细胞因子两者来增加骨破坏。
方法：在本研究中，共包括60名参与者，其中30例全身和牙周健康（对照组），30例全身健康但有牙周炎(牙周炎组)。从参与者收集牙龈沟液（GCF）和血清样品进行生化分析。采用酶联免疫吸附试验检测IL-20、肿瘤坏死因子(TNF)-α、IL1β/IL-10，RANKL/骨保护素（OPG），和基质金属蛋白酶-8(MMP8)。为了进行统计分析，独立t检验，皮尔逊相关系数，并采用卡方检验。
结果：GCFIL-20，RANKL，RANKL/OPG,血清IL-20，RANKL，RANKL/OPG,MMP-8,TNF-α,IL-1B,发现牙周炎组的IL-1β/IL-10值在统计学上明显高于对照组。发现对照组的GCFOPG和血清IL-10值在统计学上明显高于牙周炎组。两组之间的血清OPG值没有观察到统计学上的显着差异。血清IL-20值与血清RANKL呈显著正相关,RANKL/OPG,MMP-8,TNF-α,IL-1β值，和牙周临床参数。ROC曲线显示:GCFIL-20的AUC=0.788,血清IL-20的AUC=1.000。
结论：根据研究结果，发现IL-20与牙周炎有关。IL-20在牙周发病中的作用与破骨细胞生成和胶原降解有关。可以想象,IL-20可以通过影响RANKL/OPG比率和促炎细胞因子两者来增加骨破坏。
