Abstract:
Fourier-transform infrared (FTIR) spectroscopy has the potential to be used for bacterial typing and outbreak characterization. We evaluated FTIR for the characterization of an outbreak caused by Elizabethkingia miricola. During the 2020-2021 period, 26 isolates (23 clinical and 3 environmental) were collected and analyzed by FTIR (IR Biotyper) and core-genome MLST (cgMLST), in addition to antimicrobial susceptibility testing. FTIR spectroscopy and cgMLST showed that 22 of the isolates were related to the outbreak, including the environmental samples, with only one discordance between both methods. Then, FTIR is useful for E. miricola typing and can be easily implemented in the laboratory.
摘要:
傅里叶变换红外(FTIR)光谱具有用于细菌分型和爆发表征的潜力。我们评估了FTIR以表征由Elizabethkingiamiricola引起的爆发。在2020-2021年期间,收集26个分离株(23个临床和3个环境),并通过FTIR(IRBiotyper)和核心基因组MLST(cgMLST)进行分析,除了抗菌药物敏感性测试。FTIR光谱和cgMLST显示,其中22株与疫情有关,包括环境样本,两种方法之间只有一个不一致。然后,FTIR可用于E.miricola分型,可以在实验室中轻松实现。