PDF(Pubmed)
Abstract：
BACKGROUND: Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically, culturing tools are needed to propagate and shape microbial communities, to obtain desired properties, and to exploit them. Previous work suggested that micro-confinement and segregation of microorganisms using invert (water-in-oil, w/o) emulsion broth can shape communities during propagation, by alleviating biotic interactions and inducing physiological changes in cultured bacteria. The present work aimed at evaluating invert emulsion and simple broth monophasic cultures for the propagation and shaping of bacterial communities derived from raw milk in a serial propagation design.
RESULTS: The monophasic setup resulted in stable community structures during serial propagation, whereas the invert emulsion system resulted in only transiently stable structures. In addition, different communities with different taxonomic compositions could be obtained from a single inoculum. Furthermore, the implementation of invert emulsion systems has allowed for the enrichment of less abundant microorganisms and consequently facilitated their isolation on culture agar plates.
CONCLUSIONS: The monophasic system enables communities to be propagated in a stable manner, whereas the invert emulsion system allowed for the isolation of less abundant microorganisms and the generation of diverse taxonomic compositions from a single inoculum.
RESULTS: The monophasic setup resulted in stable community structures during serial propagation, whereas the invert emulsion system resulted in only transiently stable structures. In addition, different communities with different taxonomic compositions could be obtained from a single inoculum. Furthermore, the implementation of invert emulsion systems has allowed for the enrichment of less abundant microorganisms and consequently facilitated their isolation on culture agar plates.
CONCLUSIONS: The monophasic system enables communities to be propagated in a stable manner, whereas the invert emulsion system allowed for the isolation of less abundant microorganisms and the generation of diverse taxonomic compositions from a single inoculum.
摘要：
背景：微生物群落在不同领域具有重要的生物技术潜力，然而,这些社区的工程和传播仍然面临着知识和诀窍的差距。更具体地说,需要培养工具来繁殖和塑造微生物群落，为了获得所需的性能，并利用他们。以前的工作表明，微生物的微限制和分离使用转化（油包水，w/o)乳化肉汤在繁殖过程中可以形成群落，通过减轻生物相互作用和诱导培养细菌的生理变化。目前的工作旨在评估反相乳液和简单的肉汤单相培养物，以在连续繁殖设计中繁殖和塑造来自生乳的细菌群落。
结果：单相设置在连续传播过程中导致稳定的群落结构，而反相乳液系统仅产生暂时稳定的结构。此外,可以从单个接种物中获得具有不同分类组成的不同群落。此外，倒置乳液系统的实施允许富集较少的微生物，因此促进了它们在培养琼脂平板上的分离。
结论：单相系统使社区能够以稳定的方式传播，而反相乳液系统则可以分离出数量较少的微生物，并从单个接种物中产生各种分类学组成。
结果：单相设置在连续传播过程中导致稳定的群落结构，而反相乳液系统仅产生暂时稳定的结构。此外,可以从单个接种物中获得具有不同分类组成的不同群落。此外，倒置乳液系统的实施允许富集较少的微生物，因此促进了它们在培养琼脂平板上的分离。
结论：单相系统使社区能够以稳定的方式传播，而反相乳液系统则可以分离出数量较少的微生物，并从单个接种物中产生各种分类学组成。
