RESULTS: We herein developed associative substrate-based DNAzymes (ASBDs). Cyanine dyes were selected as associative substrates (ASs) due to their binding competency with G4/hemin DNAzymes. With respect to DSBDs, ASBDs needed only low dosage (∼10 μM) of ASs to be able to cause a rapid and visible substrate conversion. In addition, since cyanine dyes are NIR dyes with high extinction coefficients and their conversion products have absorption bands at shorter wavelength. Therefore, a colorimetric ratio response can be developed to follow activities of G4/hemin DNAzymes with competency to tolerate fluctuation of experimental factors and background. In particular, herein developed ASBDs can endure somewhat concentration fluctuation of H2O2. ASBDs are able to cowork with other enzymes (for example, glucose oxidase) to realize cascade sensing.
CONCLUSIONS: The developed ASBDs can operate at low dosage of substrates with a colorimetric ratio response and can overcome the drawbacks met in DSBDs. We expect that, by designing ASs with fruitful color panel in the future, our work will inspire more interesting in developing environment-benign and low-carbon G4/hemin DNAzymes and desired colorful high-performance sensors.
结果:我们在此开发了基于缔合底物的DNA酶(ASBD)。选择花青染料作为缔合底物(AS),因为它们与G4/血红素DNA酶的结合能力。关于DSBD,ASBD只需要低剂量(~10μM)的AS就能够引起快速和可见的底物转化。此外,因为花青染料是具有高消光系数的近红外染料并且它们的转化产物在较短波长处具有吸收带。因此,可以开发比色比反应以跟踪G4/血红素DNA酶的活性,具有耐受实验因素和背景的波动的能力。特别是,本文开发的ASBD可以承受一定程度的H2O2浓度波动。ASBD能够与其他酶协同工作(例如,葡萄糖氧化酶)实现级联传感。
结论:开发的ASBD可以在低剂量的底物下操作,具有比色比响应,并且可以克服DSBD中遇到的缺点。我们期望,通过在未来设计具有丰富色彩面板的AS,我们的工作将激发更有趣的开发环境良性和低碳G4/血红素DNAzymes和所需的丰富多彩的高性能传感器。