PDF(Pubmed)
Abstract：
UNASSIGNED: The aim of this study is to compare the diagnostic value of metagenomic next-generation sequencing (mNGS) vs. conventional culture methods (CM) in chronic infection and acute infection.
UNASSIGNED: We retrospectively analyzed the bronchoalveolar lavage fluid (BALF) of 88 patients with acute infection and 105 patients with chronic infection admitted to three hospitals from 2017 to 2022.
UNASSIGNED: The results showed that the sensitivity and specificity of mNGS were higher than those of CM. The number of patients who changed the antibiotic treatment in the mNGS positive group was larger than that of patients in the mNGS negative group in both the acute infection group (60.5 vs. 28.0%, P = 0.0022) and chronic infection group (46.2 vs. 22.6%, P = 0.01112). High levels of temperature (OR: 2.02, 95% CI: 1.18-3.70, P: 0.015), C-reactive protein (CRP) (OR: 15, 95% CI: 2.74-280.69, P: 0.011), neutrophil count (OR: 3.09, 95% CI: 1.19-8.43, P: 0.023), and low levels of lymphocyte count (OR: 3.43, 95% CI:1.26-10.21, P: 0.020) may lead to positive mNGS results in the acute infection group while no significant factor was identified to predict positive results in the chronic infection group.
UNASSIGNED: mNGS could provide useful guidance on antibiotic strategies in infectious diseases and may be more valuable for the diagnosis and treatment of acute infection vs. chronic infection.
UNASSIGNED: We retrospectively analyzed the bronchoalveolar lavage fluid (BALF) of 88 patients with acute infection and 105 patients with chronic infection admitted to three hospitals from 2017 to 2022.
UNASSIGNED: The results showed that the sensitivity and specificity of mNGS were higher than those of CM. The number of patients who changed the antibiotic treatment in the mNGS positive group was larger than that of patients in the mNGS negative group in both the acute infection group (60.5 vs. 28.0%, P = 0.0022) and chronic infection group (46.2 vs. 22.6%, P = 0.01112). High levels of temperature (OR: 2.02, 95% CI: 1.18-3.70, P: 0.015), C-reactive protein (CRP) (OR: 15, 95% CI: 2.74-280.69, P: 0.011), neutrophil count (OR: 3.09, 95% CI: 1.19-8.43, P: 0.023), and low levels of lymphocyte count (OR: 3.43, 95% CI:1.26-10.21, P: 0.020) may lead to positive mNGS results in the acute infection group while no significant factor was identified to predict positive results in the chronic infection group.
UNASSIGNED: mNGS could provide useful guidance on antibiotic strategies in infectious diseases and may be more valuable for the diagnosis and treatment of acute infection vs. chronic infection.
摘要：
■本研究的目的是比较宏基因组下一代测序（mNGS）与慢性感染和急性感染的常规培养方法（CM）。
■我们回顾性分析了2017年至2022年三所医院收治的88例急性感染患者和105例慢性感染患者的支气管肺泡灌洗液（BALF）。
■结果表明，mNGS的敏感性和特异性均高于CM。在两个急性感染组中，mNGS阳性组改变抗生素治疗的患者人数均大于mNGS阴性组的患者（60.5vs.28.0%,P=0.0022）和慢性感染组（46.2vs.22.6%,P=0.01112)。高温（OR：2.02，95％CI：1.18-3.70，P：0.015），C反应蛋白（CRP）（OR：15，95％CI：2.74-280.69，P：0.011），中性粒细胞计数(OR:3.09,95%CI:1.19-8.43,P:0.023),淋巴细胞计数水平低（OR：3.43，95％CI：1.26-10.21，P：0.020）可能导致急性感染组mNGS结果阳性，而在慢性感染组中没有发现预测阳性结果的重要因素组。
■mNGS可以为感染性疾病的抗生素策略提供有用的指导，并且可能对急性感染的诊断和治疗更有价值。慢性感染。
■我们回顾性分析了2017年至2022年三所医院收治的88例急性感染患者和105例慢性感染患者的支气管肺泡灌洗液（BALF）。
■结果表明，mNGS的敏感性和特异性均高于CM。在两个急性感染组中，mNGS阳性组改变抗生素治疗的患者人数均大于mNGS阴性组的患者（60.5vs.28.0%,P=0.0022）和慢性感染组（46.2vs.22.6%,P=0.01112)。高温（OR：2.02，95％CI：1.18-3.70，P：0.015），C反应蛋白（CRP）（OR：15，95％CI：2.74-280.69，P：0.011），中性粒细胞计数(OR:3.09,95%CI:1.19-8.43,P:0.023),淋巴细胞计数水平低（OR：3.43，95％CI：1.26-10.21，P：0.020）可能导致急性感染组mNGS结果阳性，而在慢性感染组中没有发现预测阳性结果的重要因素组。
■mNGS可以为感染性疾病的抗生素策略提供有用的指导，并且可能对急性感染的诊断和治疗更有价值。慢性感染。
