Abstract：
Minimal residual disease refers to a leukemia cell population that is resistant to chemotherapy or radiotherapy and leads to disease relapse. The assessment of MRD is crucial for making an accurate prognosis of the disease and for the choice of optimal treatment strategy. Here, we review the advantages and disadvantages of the available genetic and phenotypic methods and focus on the multiparametric flow cytometry as a promising method with greater sensitivity, speed, and standardization options. In addition, we discuss how the application of automated data analysis outweighs the use of complex combinations of windows and gates in classical analysis, thus eliminating subjective evaluation.
摘要：
微小残留病是指对化疗或放疗有抗性并导致疾病复发的白血病细胞群。MRD的评估对于做出疾病的准确预后和选择最佳治疗策略至关重要。这里,我们回顾了可用的遗传和表型方法的优缺点，并将重点放在多参数流式细胞术作为一种有前途的方法，具有更高的灵敏度，速度,标准化选项。此外,我们讨论了自动数据分析的应用如何胜过经典分析中窗户和大门的复杂组合的使用，从而消除了主观评价。
