Abstract：
Borna disease (BD) associated with a peracute bacterial septicaemia with Escherichia coli was diagnosed in an adult female, naturally infected, free-ranging Eurasian beaver of the subspecies Castor fiber albicus, clinically characterized by weight loss, depression, weakness and gurgled peristaltic sounds. The beaver was euthanized humanely. Necropsy and light microscopy revealed a non-purulent meningoencephalitis with typical mononuclear perivascular cuffs and parenchymal infiltrates. The diagnosis of BD was confirmed by detection of viral antigen and RNA by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). The PCR product was sequenced and cluster analysis revealed a close relationship between endemic clusters in Saxony-Anhalt. This is the first report of naturally occurring BD in a free-ranging Eurasian beaver.
摘要：
在成年女性中诊断出与大肠杆菌的急性细菌性败血症相关的博尔纳病（BD），自然感染,自由放养的欧亚海狸，临床特征为体重减轻，抑郁症,虚弱和蠕动的声音。海狸被人道地安乐死。尸检和光学显微镜检查显示非化脓性脑膜脑炎，具有典型的单核血管周围袖带和实质浸润。通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测病毒抗原和RNA来确认BD的诊断。对PCR产物进行了测序，聚类分析揭示了萨克森-安哈尔特州特有簇之间的密切关系。这是自由放养的欧亚海狸中自然发生的BD的第一份报告。
