Abstract：
Garlic-derived exosome-like nanovesicles (GELNs) could function in interspecies communication and may serve as natural therapeutics to regulate the inflammatory response or as nanocarriers to efficiently deliver specific drugs. Staphylococcus aureus (S. aureus) is able to hide within host cells to evade immune clearance and antibiotics, leading to life-threatening infections. On-site detection and efficient treatment of intracellular S. aureus infection in wounds remain challenging. Herein, we report a thermosensitive, injectable, visible GELNs-based wound dressing, Van@GELNs/F127 hydrogel (gel Van@GELNs), which is H2O2-responsive and can slowly release vancomycin into host cells forS. aureus infection visualization and treatment in wounds. GELNs show inherent antibacterial activity, which is significantly enhanced after loading vancomycin. Both GELNs and Van@GELNs have the ability to be internalized by cells, so Van@GELNs are more effective than free vancomycin in killing S. aureus in RAW 264.7 macrophages. When applied to an S. aureus-infected wound on a mouse, the colorless HRP&ABTS/Van@GELNs/F127 solution immediately changes to a green hydrogel and shows better therapeutic effect than vancomycin. Thus, direct visualization by the naked eye and effective treatment of S. aureus infection in wounds are achieved by gel Van@GELNs. We anticipate gel Van@GELNs be applied for the theranostics of S. aureus infection diseases in the clinic in the near future.
摘要：
大蒜衍生的外泌体样纳米囊泡（GELN）可以在种间通讯中起作用，并且可以作为天然治疗剂来调节炎症反应或作为纳米载体来有效地递送特定药物。金黄色葡萄球菌（S。金黄色葡萄球菌）能够隐藏在宿主细胞内以逃避免疫清除和抗生素，导致危及生命的感染.伤口中细胞内金黄色葡萄球菌感染的现场检测和有效治疗仍然具有挑战性。在这里，我们报告了一个热敏物，可注射,可见的基于GELNs的伤口敷料，Van@GELNs/F127水凝胶(gelVan@GELNs),具有H2O2响应性，可以将万古霉素缓慢释放到宿主细胞中。伤口中金黄色葡萄球菌感染的可视化和治疗。凝胶显示出固有的抗菌活性，加载万古霉素后显着增强。GELN和Van@GELN都具有被细胞内化的能力，因此，Van@GELN比游离万古霉素更有效地杀死RAW264.7巨噬细胞中的金黄色葡萄球菌。当应用于小鼠感染金黄色葡萄球菌的伤口时，无色HRP&ABTS/Van@GELNs/F127溶液立即变为绿色水凝胶,显示出比万古霉素更好的治疗效果。因此，凝胶Van@GELNs可通过肉眼直接观察和有效治疗伤口中的金黄色葡萄球菌感染。我们预计凝胶Van@GELNs将在不久的将来用于临床上金黄色葡萄球菌感染疾病的治疗。
