Abstract：
The processing of traditional Chinese medicine (TCM) plays an important role in the clinical application, which usually has the function of \"increasing efficiency and reducing toxicity\". Polygonum multiflorum (PM) has been reported to induce hepatotoxicity, while it is believed that the toxicity is reduced after processing. Studies have shown that the hepatotoxicity of PM is closely related to the changes in chemical components before and after processing. However, there is no comprehensive investigation on the chemical changes of PM during the processing progress. In this research, we established a comprehensive method to profile both small molecule compounds and polysaccharides from raw and different processed PM samples. In detail, an online two-dimensional liquid chromatography coupled with quadrupole-orbitrap mass spectrometry (2D-LC/Q-Orbitrap MS) was utilized to investigate the small molecules, and a total of 150 compounds were characterized successfully. After multivariate statistical analysis, 49 differential compounds between raw and processed products were screened out. Furthermore, an accurate and comprehensive method for quantification of differential compounds in PM samples was established based on ultra-high performance liquid chromatography/Q-Orbitrap-MS (UHPLC/Q-Orbitrap-MS) within 16 min. In addition, the changes of polysaccharides in different PM samples were analyzed, and it was found that the addition of black beans and steaming times would affect the content and composition of polysaccharides in PM significantly. Our work provided a reference basis for revealing the scientific connotation of the processing technology and increasing the quality control and safety of PM.
摘要：
中药炮制在临床应用中起着重要作用,通常具有“提高效率和减少毒性”的功能。据报道，何首乌（PM）可诱导肝毒性，而据信加工后毒性降低。研究表明，PM的肝毒性与加工前后化学成分的变化密切相关。然而,没有对加工过程中PM的化学变化进行全面的调查。在这项研究中,我们建立了一个全面的方法来分析小分子化合物和多糖从原始和不同的处理PM样品。详细来说,利用在线二维液相色谱与四极-轨道阱质谱联用(2D-LC/Q-OrbitrapMS)来研究小分子,共150个化合物被成功表征。经过多元统计分析，筛选出49种原料和加工产品之间的差异化合物。此外，基于超高效液相色谱/Q-Orbitrap-MS（UHPLC/Q-Orbitrap-MS），在16min内建立了准确，全面的定量PM样品中差异化合物的方法。此外,分析了不同PM样品中多糖的变化,研究发现，黑豆的添加和蒸时间会显著影响PM中多糖的含量和组成。为揭示加工工艺的科学内涵、加强PM的质量控制和安全性提供了参考依据。
