Abstract：
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) is a deadly disease due to the lack of early detection. Because chronic pancreatitis (CP) patients are a high-risk group for pancreatic cancer, this study aimed to assess the differential miRNA profile in pancreatic tissue of patients with CP and pancreatic cancer.
METHODS: MiRNAs were isolated from formalin-fixed paraffin-embedded pancreatic tissue of 22 PDAC patients, 18 CP patients, and 10 normal pancreatic tissues from autopsy (C) cases and processed for next-generation sequencing. Known and novel miRNAs were identified and analyzed for differential miRNA expression, target prediction, and pathway enrichment between groups.
RESULTS: Among the miRNAs identified, 166 known and 17 novel miRNAs were found exclusively in PDAC tissues, while 106 known and 10 novel miRNAs were found specifically in CP tissues. The pathways targeted by PDAC-specific miRNAs and differentially expressed miRNAs between PDAC versus CP tissues and PDAC versus control tissues were the proteoglycans pathway, Hippo signaling pathway, adherens junction, and transforming growth factor-β signaling pathway.
CONCLUSIONS: This study resulted in a set of exclusive and differentially expressed miRNAs in PDAC and CP can be assessed for their diagnostic value. In addition, studying the role of miRNA-target gene interactions in carcinogenesis may open new therapeutic avenues.
METHODS: MiRNAs were isolated from formalin-fixed paraffin-embedded pancreatic tissue of 22 PDAC patients, 18 CP patients, and 10 normal pancreatic tissues from autopsy (C) cases and processed for next-generation sequencing. Known and novel miRNAs were identified and analyzed for differential miRNA expression, target prediction, and pathway enrichment between groups.
RESULTS: Among the miRNAs identified, 166 known and 17 novel miRNAs were found exclusively in PDAC tissues, while 106 known and 10 novel miRNAs were found specifically in CP tissues. The pathways targeted by PDAC-specific miRNAs and differentially expressed miRNAs between PDAC versus CP tissues and PDAC versus control tissues were the proteoglycans pathway, Hippo signaling pathway, adherens junction, and transforming growth factor-β signaling pathway.
CONCLUSIONS: This study resulted in a set of exclusive and differentially expressed miRNAs in PDAC and CP can be assessed for their diagnostic value. In addition, studying the role of miRNA-target gene interactions in carcinogenesis may open new therapeutic avenues.
摘要：
背景：由于缺乏早期检测，胰腺导管腺癌（PDAC）是一种致命的疾病。因为慢性胰腺炎（CP）患者是胰腺癌的高危人群，这项研究旨在评估CP和胰腺癌患者胰腺组织中差异的miRNA谱。
方法：从22例PDAC患者福尔马林固定石蜡包埋的胰腺组织中分离MiRNAs，18例CP患者，和10个来自尸检（C）病例的正常胰腺组织，并进行下一代测序。已知和新的miRNA被鉴定和分析差异miRNA表达,目标预测，和群体之间的途径富集。
结果：在鉴定的miRNA中，在PDAC组织中发现了166个已知的和17个新的miRNA，而在CP组织中发现了106个已知的miRNA和10个新的miRNA。PDAC相对于CP组织和PDAC相对于对照组织之间的PDAC特异性miRNAs和差异表达miRNAs的靶向途径是蛋白聚糖途径,河马信号通路,附着者接合处，转化生长因子-β信号通路。
结论：这项研究导致PDAC和CP中一组排他性和差异表达的miRNA可以评估其诊断价值。此外,研究miRNA-靶基因相互作用在癌变中的作用可能开辟新的治疗途径.
方法：从22例PDAC患者福尔马林固定石蜡包埋的胰腺组织中分离MiRNAs，18例CP患者，和10个来自尸检（C）病例的正常胰腺组织，并进行下一代测序。已知和新的miRNA被鉴定和分析差异miRNA表达,目标预测，和群体之间的途径富集。
结果：在鉴定的miRNA中，在PDAC组织中发现了166个已知的和17个新的miRNA，而在CP组织中发现了106个已知的miRNA和10个新的miRNA。PDAC相对于CP组织和PDAC相对于对照组织之间的PDAC特异性miRNAs和差异表达miRNAs的靶向途径是蛋白聚糖途径,河马信号通路,附着者接合处，转化生长因子-β信号通路。
结论：这项研究导致PDAC和CP中一组排他性和差异表达的miRNA可以评估其诊断价值。此外,研究miRNA-靶基因相互作用在癌变中的作用可能开辟新的治疗途径.
