Abstract：
The N-acyl-homoserine lactones (AHLs)-mediated quorum-sensing (QS) system played a crucial role in regulating biological nitrogen removal and biofilm formation. However, the regulatory role of AHLs on nitrogen removal bacteria in high salinity environment has remained unclear. This study evaluated the roles and release patterns of AHLs in Vibrio sp. LV-Q1 under high salinity condition. Results showed that Vibrio sp. primarily secretes five AHLs, and the AHLs activity is strongly correlated with the bacterial density. Exogenous C10-HSL and 3OC10-HSL were found to significantly enhance ammonium removal, while making a minor contribution to the growth rate. Both the C10-HSL and 3OC10-HSL promoted the biofilm formation of Vibrio sp. with an enhancement of 1.64 and 1.78 times, respectively. The scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM) observations confirmed the biofilm-enhancing effect of AHLs. Further analysis revealed that AHLs significantly improved bacterial self-aggregation and motility, as well as the level of extracellular polymeric substances (EPS). These findings provide significant guidance on construction of nitrification system at high salinity.
摘要：
N-酰基高丝氨酸内酯（AHLs）介导的群体感应（QS）系统在调节生物脱氮和生物膜形成中起着至关重要的作用。然而,高盐度环境中AHLs对脱氮菌的调节作用尚不清楚。本研究评估了AHLs在弧菌中的作用和释放模式。高盐度条件下的LV-Q1。结果表明，弧菌。主要分泌5种AHL,AHLs活性与细菌密度密切相关。发现外源C10-HSL和3OC10-HSL显着增强铵的去除，同时对增长率的贡献很小。C10-HSL和3OC10-HSL均促进弧菌的生物膜形成。增强了1.64和1.78倍，分别。扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）观察证实了AHLs的生物膜增强作用。进一步的分析表明，AHLs显着改善细菌的自聚集和运动，以及胞外聚合物（EPS）的水平。这些研究结果为高盐度硝化系统的建设提供了重要的指导。
