Abstract：
Cutaneous pythiosis is a life-threatening infectious disease. Low-level laser therapy (LLLT) and ozone (O3) have been used individually in the treatment of infected wounds. The goals of the study were a) to characterize the antimicrobial action of the photo-ozone therapy (LLLT-O3) against equine Pythium insidiosum, and b) to assess the cytotoxic potential of the LLLT-O3 in keratinocytes. Specimens of pathogen were isolated from 10 horses. After culturing, 120 hyphae plugs were distributed among four groups (n=30 hyphae plugs/group): LLLT (laser irradiation for 160 sec;), O3 (exposition to O3 for 15 min;), LLLT-O3 (LLLT and O3 treatments in sequence) and control (untreated plugs). The hyphae growth was measured during the first 14 days post-treatment. Where there was an absence of hyphae growth, the plug was recultured for an additional 7 days. The cytotoxic potential of the treatments against HaCaT keratinocytes was assessed by colorimetric assays. The LLLT-O3 and O3 treatments inactivated, respectively, 92.3% (28/30) and 30% (9/30) of the samples. No growth was detected after 7 days reculture of inactivated hyphae plugs on new media. Hyphae growth was visualized in 100% of the control and LLLT hyphae plugs. The viability of HaCaT cells was not affected by the isolated treatments (LLLT and O3), while the LLLT-O3 showed slight cytotoxic effect (20%) when compared to the control group (P<0.05). Photo-ozone therapy inactivated equine P. insidiosum hyphae with minimal cytotoxicity in skin cells in vitro.
摘要：
皮肤脓毒血症是一种危及生命的感染性疾病。低水平激光治疗（LLLT）和臭氧（O3）已分别用于治疗感染的伤口。该研究的目标是a）表征光臭氧疗法（LLLT-O3）对马腐霉的抗菌作用，和b)评估LLLT-O3在角质形成细胞中的细胞毒性潜力。从10匹马分离病原体标本。培养后,120个菌丝塞分布在四组（n=30个菌丝塞/组）中：LLLT（激光照射160秒；），O3（暴露于O315分钟；），LLLT-O3(依次进行LLLT和O3处理)和对照(未处理的塞子)。在治疗后的前14天测量菌丝生长。在没有菌丝生长的地方，该堵塞物再培养7天.通过比色测定评估针对HaCaT角质形成细胞的处理的细胞毒性潜力。LLLT-O3和O3处理失活，分别,92.3%(28/30)和30%(9/30)的样品。在新培养基上重新培养灭活的菌丝栓7天后未检测到生长。在100%的对照和LLLT菌丝栓中观察到菌丝生长。HaCaT细胞的活力不受分离处理（LLLT和O3）的影响，与对照组相比，LLLT-O3表现出轻微的细胞毒性作用（20％）（P<0.05）。光臭氧疗法在体外对皮肤细胞具有最小的细胞毒性的灭活马。
