PDF(Pubmed)
Abstract：
Knee osteoarthritis (OA), an age-related degenerative disease characterized by severe pain and disability, is treated using polynucleotides (PNs) and hyaluronic acid (HA). The intra-articular (IA) injection of HA has been studied extensively in both animal models and in humans; however, the efficacy and mechanisms of action remain unclear. In addition, there has been a paucity of research regarding the use of PN alone or in combination with HA in OA. To investigate the effect of the combined injection of PN and HA in vivo, pathological and behavioral changes were assessed in an OA model. Anterior cruciate ligament transection and medial meniscectomy were performed in Sprague-Dawley rats to create the OA animal model. The locomotor activity improved following PNHA injection, while the OARSI grade improved in the medial tibia and femur. In mild OA, TNFα levels decreased histologically in the PN, HA, and PNHA groups but only the PNHA group showed behavioral improvement in terms of distance. In conclusion, PNHA exhibited anti-inflammatory effects during OA progression and improved locomotor activity regardless of the OARSI grade.
摘要：
膝骨关节炎（OA），一种以严重疼痛和残疾为特征的与年龄相关的退行性疾病，使用多核苷酸(PNs)和透明质酸(HA)处理。关节内（IA）注射HA已经在动物模型和人类中进行了广泛的研究；然而，疗效和作用机制尚不清楚.此外,关于在OA中单独使用PN或与HA联合使用的研究很少。探讨体内联合注射PN和HA的效果。在OA模型中评估病理和行为变化.在Sprague-Dawley大鼠中进行前交叉韧带横断和内侧半月板切除术以创建OA动物模型。PNHA注射后运动活动改善，而胫骨和股骨内侧的OARSI等级有所改善。在轻度OA中，TNFα水平在PN的组织学上降低，HA,和PNHA组，但只有PNHA组在距离方面表现出行为改善。总之,无论OARSI等级如何，PNHA在OA进展期间均表现出抗炎作用，并改善了运动活性。
