PDF(Pubmed)
Abstract：
In the artificial breeding of Pampus argenteus (Euphrasen, 1788), female fish spawn before male release sperm, which indicates rapid ovarian development. In fish, aromatase is responsible for converting androgens into estrogens and estrogen plays a crucial role in ovarian development. In this study, we aimed to investigate the potential role of brain-type and ovarian-type aromatase to study the rapid ovarian development mechanism. The results showed that cyp19a1a was mainly expressed in the ovary and could be classified as the ovarian type, whereas cyp19a1b could be considered as the brain type for its expression was mainly in the brain. During ovarian development, the expression of cyp19a1a in the ovary significantly increased from stage IV to stage V and Cyp19a1a signals were present in the follicle cells, while cyp19a1b expression in the pituitary gland decreased from stage IV to stage V. To further investigate the function of Cyp19a1a, recombinant Cyp19a1a (rCyp19a1a) was produced and specific anti-Cyp19a1a antiserum was obtained. The expressions of cyp19a1a, estrogen receptors 2 alpha (esr2a), and androgen receptor alpha (arα) were significantly upregulated in the presence of rCyp19a1a. Meanwhile, cyp19a1a was expressed significantly after E2 treatment in both ovarian and testicular tissue culture. Taken together, we found two forms of aromatase in silver pomfret. The ovarian-type aromatase might play an important role in ovarian differentiation and maturation, and participate in E2 synthesis through co-regulation with esr2a. The brain-type aromatase cyp19a1b might be involved in the regulation of both brain and gonadal development.
摘要：
在Pampusargenteus（Euphrasen，1788),雌性鱼在雄性释放精子之前产卵，这表明卵巢发育迅速。在鱼中，芳香化酶负责将雄激素转化为雌激素，而雌激素在卵巢发育中起着至关重要的作用。在这项研究中,我们旨在探讨脑型和卵巢型芳香化酶的潜在作用，以研究卵巢快速发育机制。结果表明，cyp19a1a主要在卵巢中表达，可以归类为卵巢型，而cyp19a1b可以认为是脑型，因为它的表达主要在脑中。在卵巢发育过程中,从IV期到V期，Cyp19a1a在卵巢中的表达显着增加，Cyp19a1a信号存在于卵泡细胞中，而cyp19a1b在垂体中的表达从IV期到V期降低。为了进一步研究Cyp19a1a的功能，产生重组Cyp19a1a(rCyp19a1a)并获得特异性抗Cyp19a1a抗血清。cyp19a1a的表达，雌激素受体2α（esr2a），在rCyp19a1a存在下，雄激素受体α（arα）显着上调。同时,E2处理后，cyp19a1a在卵巢和睾丸组织培养中均显着表达。一起来看,我们在银鱼中发现了两种形式的芳香化酶。卵巢型芳香化酶可能在卵巢的分化和成熟中起重要作用,并通过与esr2a共调参与E2的合成。脑型芳香化酶cyp19a1b可能参与脑和性腺发育的调节。
