PDF(Pubmed)
Abstract：
Some South American countries have ancient traditions that may pose legal problems, such as the consumption of coca leaves, as this can provide positive results for cocaine use after the analysis of biological samples. For this reason, it is necessary to find specific markers that help differentiate legal from illegal consumption, such as tropacocaine, cinnamoylcocaine, and especially hygrine and cuscohygrine. In this work, two techniques for collecting biological samples are compared: the Quantisal® Oral Fluid collection device and passive drooling. Once the samples were collected, they were subjected to solid-phase extraction for subsequent injection into GC-MS. Different validation parameters included in international guides have been studied to evaluate whether the proposed method is valid for the defined purpose, placing special emphasis on the study of the matrix effect and little value on GC-MS analyses. With respect to this parameter, an increase in the signal was found for CUS and t-CIN, but it was not significant for the rest of the substances studied. The recoveries have varied significantly depending on the way of working, being higher when working with standardized areas. After carrying out work with the oral fluid samples collected from laboratory volunteers, the method was applied to two real samples. The results obtained support the need for further research to overcome certain limitations presented by the device.
摘要：
一些南美国家有古老的传统，可能会带来法律问题，比如食用古柯叶，因为这可以为生物样本分析后的可卡因使用提供积极的结果。出于这个原因,有必要找到有助于区分合法消费和非法消费的特定标记，如替帕柯卡因，肉桂可卡因,尤其是Hygrine和cuscohygrine。在这项工作中,比较了两种收集生物样品的技术：Quantisal®口腔液体收集装置和被动流口水。一旦样本被收集,将它们进行固相萃取，随后注入GC-MS。研究了国际指南中包含的不同验证参数，以评估所提出的方法是否对定义的目的有效，特别强调基体效应的研究，对GC-MS分析的价值不大。关于此参数，CUS和t-CIN的信号增加，但对其他研究物质并不重要。回收率因工作方式而异，在标准化领域工作时更高。在对实验室志愿者收集的口腔液样本进行工作后，该方法应用于两个实际样品。获得的结果支持需要进一步研究以克服该装置所呈现的某些限制。
