PDF(Pubmed)
Abstract：
An in-depth understanding of Se uptake and metabolism in plants is necessary for developing Se biofortification strategies. Thus, hydroponic experiments were conducted to investigate the associated processes and mechanisms of organic Se (selenomethionine (SeMet) and selenomethionine-oxide (SeOMet)) uptake, translocation, transformation and their interaction in wheat, in comparison to inorganic Se. The results showed that Se uptake by the roots and the root-to-shoot translocation factor under the SeMet treatment were higher than those under the selenite, selenate and SeOMet treatments. The uptake and translocation of SeMet were higher than those of SeOMet within 72 h, although the differences gradually narrowed with time. The uptake of SeMet and SeOMet was also sensitive to the aquaporin inhibitor: AgNO3 addition resulted in 99.5% and 99.9% inhibitions of Se in the root in the SeMet and SeOMet treatments, respectively. Once absorbed by the root, they rapidly assimilated to other Se forms, and SeMet and Se-methyl-selenocysteine (MeSeCys) were the dominant species in SeMet- and SeOMet-treated plants, while notably, an unidentified Se form was also found in the root and xylem sap under the SeMet treatment. In addition, within 16 h, SeOMet inhibited the uptake and translocation of SeMet, while the inhibition was weakened with longer treatment time. Taken together, the present study provides new insights for the uptake and transformation processes of organic Se within plants.
摘要：
深入了解植物中硒的吸收和代谢对于制定硒生物强化策略是必要的。因此，进行了水培实验，以研究有机Se（硒代蛋氨酸（SeMet）和硒代蛋氨酸-氧化物（SeOMet））吸收的相关过程和机理，易位,小麦中的转化及其相互作用，与无机硒相比。结果表明，SeMet处理下根对硒的吸收和根至茎的转运因子高于亚硒酸盐处理下的硒。硒酸盐和SeOMet治疗。在72h内，SeMet的摄取和易位高于SeOMet，尽管差异随着时间逐渐缩小。SeMet和SeOMet的摄取也对水通道蛋白抑制剂敏感：在SeMet和SeOMet处理中，AgNO3的添加导致根中Se的抑制分别为99.5％和99.9％，分别。一旦被根吸收，它们迅速被其他硒形式同化，SeMet和Se-甲基-硒代半胱氨酸（MeSeCys）是SeMet和SeOMet处理的植物中的优势物种，值得注意的是,在SeMet处理下，在根和木质部汁液中也发现了一种未知的Se形式。此外,在16小时内，SeOMet抑制SeMet的摄取和转运,而抑制作用随着治疗时间的延长而减弱。一起来看,本研究为植物体内有机硒的吸收和转化过程提供了新的见解。
