PDF(Pubmed)
Abstract：
The development of adaptation strategies for crops under ever-changing climate conditions is a critically important food security issue. Studies of barley responses to ionising radiation showed that this evolutionarily ancient stress factor can be successfully used to identify molecular pathways involved in adaptation to a range of abiotic stressors. In order to identify potential molecular contributors to abiotic stress resilience, we examined the transcriptomic profiles of barley seedlings after exposure to γ-rays, electrons, and protons. A total of 553 unique differentially expressed genes with increased expression and 124 with decreased expression were detected. Among all types of radiation, the highest number of differentially expressed genes was observed in electron-irradiated samples (428 upregulated and 56 downregulated genes). Significant upregulation after exposure to the three types of radiation was shown by a set of ROS-responsive genes, genes involved in DNA repair, cell wall metabolism, auxin biosynthesis and signalling, as well as photosynthesis-related genes. Most of these genes are known to be involved in plant ROS-mediated responses to other abiotic stressors, especially with genotoxic components, such as heavy metals and drought. Ultimately, the modulation of molecular pathways of plant responses to ionising radiation may be a prospective tool for stress tolerance programmes.
摘要：
在不断变化的气候条件下制定作物适应战略是一个至关重要的粮食安全问题。大麦对电离辐射的反应研究表明，这种进化上古老的胁迫因子可以成功地用于鉴定与适应一系列非生物胁迫有关的分子途径。为了确定非生物胁迫恢复力的潜在分子贡献者，我们检查了大麦幼苗暴露于γ射线后的转录组概况，电子,还有质子.检测到总共553个表达增加的独特差异表达基因和124个表达减少的差异表达基因。在所有类型的辐射中，在电子辐照样品中观察到的差异表达基因数量最多(428个上调基因和56个下调基因).一组ROS响应基因显示了暴露于三种类型辐射后的显着上调，参与DNA修复的基因，细胞壁代谢，生长素生物合成和信号，以及与光合作用相关的基因。已知这些基因中的大多数与植物ROS介导的对其他非生物应激源的反应有关。尤其是基因毒性成分，比如重金属和干旱。最终,植物对电离辐射反应的分子途径的调节可能是胁迫耐受计划的预期工具。
