PDF(Pubmed)
Abstract：
Postural Orthostatic Tachycardia Syndrome (POTS) reflects an autonomic dysfunction, which can occur as a complication to COVID-19. Our aim was to examine gastrointestinal symptoms and gut microbiota composition in patients with POTS and post-acute COVID-19 syndrome (PACS), compared with controls. POTS patients (n = 27), PACS patients (n = 32) and controls (n = 39) delivered fecal samples and completed a 4-day food diary, irritable bowel syndrome-severity scoring system (IBS-SSS), and visual analog scale for IBS (VAS-IBS). A total of 98 DNA aliquots were sequenced to an average depth of 28.3 million (M) read pairs (Illumina 2 × 150 PE) per sample. Diversity and taxonomic levels of the microbiome, as well as functional abundances were calculated for POTS and PACS groups, then compared with controls. There were several differences in taxonomic composition between POTS and controls, whereas only the abundance of Ascomycota and Firmicutes differed between PACS and controls. The clinical variables total IBS-SSS, fatigue, and bloating and flatulence significantly correlated with multiple individual taxa abundances, alpha diversity, and functional abundances. We conclude that POTS, and to a less extent PACS, are associated with differences in gut microbiota composition in diversity and at several taxonomic levels. Clinical symptoms are correlated with both alpha diversity and taxonomic and functional abundances.
摘要：
体位性心动过速综合征（POTS）反映了自主神经功能障碍，这可能是COVID-19的并发症。我们的目的是检查POTS和急性COVID-19综合征（PACS）患者的胃肠道症状和肠道菌群组成，与对照组相比。POTS患者（n=27），PACS患者（n=32）和对照组（n=39）提供粪便样本并完成4天的食物日记，肠易激综合征严重程度评分系统（IBS-SSS），IBS的视觉模拟量表(VAS-IBS)。对总共98个DNA等分试样进行测序,每个样品的平均深度为28.3百万(M)个读段对(Illumina2X150PE)。微生物组的多样性和分类水平，以及计算POTS和PACS组的功能丰度，然后与对照组进行比较。POTS和对照之间的分类组成存在一些差异，而PACS和对照之间仅子囊胚和Firmicutes的丰度不同。临床变量总IBS-SSS，疲劳,腹胀和胀气与多个个体类群丰度显着相关，阿尔法多样性，和功能丰富。我们得出结论，POTS，在较小程度上PACS，与肠道微生物群组成在多样性和几个分类学水平上的差异有关。临床症状与α多样性以及分类学和功能丰度相关。
