Abstract：
BACKGROUND: Ionic calcium (Ca2+) plays a crucial role in maintaining normal physiological and biochemical functions within the human body. Detecting the concentration of Ca2+ is of utmost significance for various purposes, including disease screening, cellular metabolism research, and evaluating drug effectiveness. However, current detection approaches such as fluorescence and colorimetry face limitations due to complex labeling techniques and the inability to track changes in Ca2+ concentration. In recent years, extensive research has been conducted in this field to explore label-free and efficient approaches.
RESULTS: In this study, a novel light-addressed potentiometric sensor (LAPS) using silicon-on-sapphire technology, has been successfully developed for Ca2+ sensing. The Ca2+-sensitive LAPS achieved a wide-range detection of Ca2+, ranging from 10-2 M to 10-7 M, with an impressive detection limit of 100 nM. These advancements are attributed to the ultra-thin silicon layer, silicon dioxide layer, and solid-state silicon rubber sensitive membrane around 6 μm. Furthermore, the sensor demonstrated the ability to dynamically monitor fluctuations in Ca2+ concentration ranging from 10-9 M to 10-2 M within a solution. Its remarkable selectivity, specificity, and long-term stability have facilitated its successful application in the detection of Ca2+ in human serum and urine.
UNASSIGNED: This work presents a Ca2+-sensitive sensor that combines a low detection limit and a wide detection range. The development represents the emergence of a label-free and rapid Ca2+ detection tool with immense prospects in home-based health monitoring, community disease screening, as well as cellular metabolism, and drug screening evaluations.
RESULTS: In this study, a novel light-addressed potentiometric sensor (LAPS) using silicon-on-sapphire technology, has been successfully developed for Ca2+ sensing. The Ca2+-sensitive LAPS achieved a wide-range detection of Ca2+, ranging from 10-2 M to 10-7 M, with an impressive detection limit of 100 nM. These advancements are attributed to the ultra-thin silicon layer, silicon dioxide layer, and solid-state silicon rubber sensitive membrane around 6 μm. Furthermore, the sensor demonstrated the ability to dynamically monitor fluctuations in Ca2+ concentration ranging from 10-9 M to 10-2 M within a solution. Its remarkable selectivity, specificity, and long-term stability have facilitated its successful application in the detection of Ca2+ in human serum and urine.
UNASSIGNED: This work presents a Ca2+-sensitive sensor that combines a low detection limit and a wide detection range. The development represents the emergence of a label-free and rapid Ca2+ detection tool with immense prospects in home-based health monitoring, community disease screening, as well as cellular metabolism, and drug screening evaluations.
摘要：
背景：离子钙（Ca2）在维持人体内正常的生理和生化功能中起着至关重要的作用。检测Ca2+的浓度对于各种目的都具有极其重要的意义。包括疾病筛查,细胞代谢研究，并评估药物的有效性。然而,由于复杂的标记技术和无法跟踪Ca2+浓度的变化，目前的检测方法如荧光和比色法面临着局限性。近年来,在这一领域进行了广泛的研究，以探索无标签和有效的方法。
结果：在这项研究中，使用蓝宝石上硅技术的新型光寻址电位传感器（LAPS），已成功开发用于Ca2+传感。Ca2+敏感型LAPS实现了对Ca2+的宽范围检测，范围从10-2M到10-7M，具有令人印象深刻的检测限为100nM。这些进步归功于超薄硅层，二氧化硅层，和6μm左右的固态硅橡胶敏感膜。此外，该传感器展示了动态监测溶液中Ca2+浓度波动的能力，范围从10-9M到10-2M。其显著的选择性，特异性，和长期稳定性促进了其在人血清和尿液中Ca2+检测中的成功应用。
■这项工作提出了一种Ca2敏感的传感器，该传感器结合了低检测极限和宽检测范围。这一发展代表了一种无标签和快速Ca2+检测工具的出现，在基于家庭的健康监测中具有巨大的前景。社区疾病筛查，以及细胞代谢，和药物筛选评估。
结果：在这项研究中，使用蓝宝石上硅技术的新型光寻址电位传感器（LAPS），已成功开发用于Ca2+传感。Ca2+敏感型LAPS实现了对Ca2+的宽范围检测，范围从10-2M到10-7M，具有令人印象深刻的检测限为100nM。这些进步归功于超薄硅层，二氧化硅层，和6μm左右的固态硅橡胶敏感膜。此外，该传感器展示了动态监测溶液中Ca2+浓度波动的能力，范围从10-9M到10-2M。其显著的选择性，特异性，和长期稳定性促进了其在人血清和尿液中Ca2+检测中的成功应用。
■这项工作提出了一种Ca2敏感的传感器，该传感器结合了低检测极限和宽检测范围。这一发展代表了一种无标签和快速Ca2+检测工具的出现，在基于家庭的健康监测中具有巨大的前景。社区疾病筛查，以及细胞代谢，和药物筛选评估。
