PDF(Pubmed)
Abstract：
Mosquito-borne diseases pose a significant threat in many Southeast Asian countries, particularly through the sylvatic cycle, which has a wildlife reservoir in forests and rural areas. Studying the composition and diversity of vectors and pathogen transmission is especially challenging in forests and rural areas due to their remoteness, limited accessibility, lack of power, and underdeveloped infrastructure. This study is based on the WHO mosquito sampling protocol, modifies technical details to support mosquito collection in difficult-to-access and resource-limited areas. Specifically, we describe the procedure for using rechargeable lithium batteries and solar panels to power the mosquito traps, demonstrate a workflow for processing and storing the mosquitoes in a -20 °C freezer, data management tools including microclimate data, and quality assurance processes to ensure the validity and reliability of the results. A pre- and post-test was utilized to measure participant knowledge levels. Additional research is needed to validate this protocol for monitoring vector-borne diseases in hard-to-reach areas within other countries and settings.
摘要：
蚊媒疾病在许多东南亚国家构成重大威胁,特别是在三级制循环中，在森林和农村地区有野生动物水库。研究媒介和病原体传播的组成和多样性在森林和农村地区尤其具有挑战性，有限的可访问性，缺乏权力,和不发达的基础设施。这项研究是基于世界卫生组织的蚊子采样协议，修改技术细节，以支持在难以进入和资源有限的地区收集蚊子。具体来说,我们描述了使用可充电锂电池和太阳能电池板为诱蚊器供电的过程，演示在-20°C冰箱中处理和储存蚊子的工作流程，数据管理工具，包括小气候数据，和质量保证过程，以确保结果的有效性和可靠性。使用前后测试来衡量参与者的知识水平。需要进行更多的研究来验证该协议，以监测其他国家和地区难以到达的地区的媒介传播疾病。
