PDF(Pubmed)
Abstract：
This study focused on examining the exact role of circulating immune cells in the development of diabetic retinopathy (DR). In vitro co-culture experiments showed that peripheral blood mononuclear cells (PBMCs) from patients with type 1 DR crucially modulated the biological functions of human retinal microvascular endothelial cells (HRMECs), consequently disrupting their normal functionality. Single-cell RNA sequencing (scRNA-seq) study revealed unique differentially expressed genes and pathways in circulating immune cells among healthy controls, non-diabetic retinopathy (NDR) patients, and DR patients. Of significance was the observed upregulation of JUND in each subset of PBMCs in patients with type 1 DR. Further studies showed that downregulating JUND in DR patient-derived PBMCs led to the amelioration of HRMEC dysfunction. These findings highlighted the notable alterations in the transcriptomic patterns of circulating immune cells in type 1 DR patients and underscored the significance of JUND as a key factor for PBMCs in participating in the pathogenesis of DR.
摘要：
这项研究的重点是检查循环免疫细胞在糖尿病视网膜病变（DR）发展中的确切作用。体外共培养实验表明，1型DR患者外周血单个核细胞（PBMC）对人视网膜微血管内皮细胞（HRMEC）的生物学功能具有重要的调节作用，从而破坏了它们的正常功能。单细胞RNA测序（scRNA-seq）研究揭示了健康对照中循环免疫细胞中独特的差异表达基因和途径。非糖尿病视网膜病变（NDR）患者，和DR患者。在1型DR患者中，观察到的每个PBMC亚群中JUND的上调具有重要意义。进一步的研究表明，在DR患者来源的PBMC中下调JUND导致HRMEC功能障碍的改善。这些发现强调了1型DR患者循环免疫细胞转录模式的显着改变，并强调了JUND作为PBMC参与DR发病机理的关键因素的重要性。
