Abstract：
Maternal protein restriction delays the differentiation of epididymal mesenchymal cells in newborn rats. However, it\'s unclear if this delay persists until the full differentiation of the epididymal epithelium at 44 days postnatal. Thus, this study aimed to assess the impact of maternal protein reduction on 44-day-old rats\' epididymal epithelium differentiation, following up on the observed delay in newborn animals. Pregnant rats were randomly divided into groups receiving normal-protein (NP - 17% protein) or low-protein (LP - 6% protein) diets during gestation and lactation. On postnatal day (PDN) 44, male offspring were euthanized, and the epididymis (NP n=10, LP n=10) was processed according to immunohistochemical techniques for the detection of aquaporin 9 (AQP9), KI-67, TP63, and ATPase. LP rats showed: a decrease in the intensity of the AQP9 reaction, an increase in cellular proliferation in the initial segment and corpus of the epididymis, an increase in basal cells in the caput and corpus epididymis, and an increase in ATPase-positive clear cells in the cauda region. These findings demonstrate that maternal protein restriction impacts cell differentiation in the epididymal epithelium of 44-day-old rats, persisting even with a normal-protein diet after weaning.
摘要：
母体蛋白限制延迟新生大鼠附睾间充质细胞的分化。然而,目前尚不清楚这种延迟是否持续到出生后44天附睾上皮完全分化。因此，本研究旨在评估母体蛋白质减少对44日龄大鼠附睾上皮分化的影响，跟进在新生动物中观察到的延迟。在妊娠和哺乳期间，将妊娠大鼠随机分为接受正常蛋白（NP-17％蛋白）或低蛋白（LP-6％蛋白）饮食的组。出生后第44天，雄性后代被安乐死，根据免疫组织化学技术处理附睾（NPn=10，LPn=10）以检测水通道蛋白9（AQP9），KI-67、TP63和ATP酶。LP大鼠显示：AQP9反应强度降低，附睾的初始节段和主体的细胞增殖增加，毛细血管和附睾中基底细胞的增加，和尾区域ATPase阳性透明细胞的增加。这些发现表明，母体蛋白限制会影响44日龄大鼠附睾上皮细胞的分化，即使在断奶后保持正常的蛋白质饮食。
