Abstract：
Marek\'s disease (MD) is a neoplastic disease that significantly affects the poultry industry. Long non-coding RNAs (lncRNAs) are crucial regulatory factors in various biological processes, including tumourigenesis. However, the involvement of novel lncRNAs in the course of MD virus (MDV) infection is still underexplored. Here, we present the first comprehensive characterization of differentially expressed lncRNAs in chicken spleen at different stages of MDV infection. A series of differentially expressed lncRNAs was identified at each stage of MDV infection through screening. Notably, our investigation revealed a novel lncRNA, lncRNA 803, which exhibited significant differential expression at different stages of MDV infection and was likely to be associated with the p53 pathway. Further analyses demonstrated that the overexpression of lncRNA 803 positively regulated the expression of p53 and TP53BP1 in DF-1 cells, leading to the inhibition of apoptosis. This is the first study to focus on the lncRNA expression profiles in chicken spleens during MDV pathogenesis. Our findings highlight the potential role of the p53-related novel lncRNA 803 in MD pathogenesis and provide valuable insights for decoding the molecular mechanism of MD pathogenesis involving non-coding RNA.RESEARCH HIGHLIGHTS Differentially expressed lncRNAs in spleens of chickens infected with Marek\'s disease virus at different stages were identified for the first time.The effects of novel lncRNA 803 on p53 pathway and apoptosis of DF-1 cells were reported for the first time.
摘要：
马立克氏病（MD）是一种肿瘤性疾病，显着影响家禽业。长链非编码RNA(lncRNAs)是各种生物过程中的关键调控因子,包括肿瘤发生。然而,新型lncRNAs参与MD病毒（MDV）的发病机制仍未得到充分研究。这里,我们首次对MDV感染不同阶段鸡脾脏中差异表达的lncRNAs进行了全面的表征。通过筛选在MDV感染的每个阶段鉴定了一系列差异表达的lncRNA。值得注意的是,我们的调查揭示了一种新的lncRNA，lncRNA803,其在MDV感染的不同阶段表现出显著差异表达,并且估计与p53途径相关。进一步分析表明，lncRNA803的过表达正调控DF-1细胞中p53和TP53BP1的表达，导致细胞凋亡的抑制。这是第一个专注于MDV发病过程中鸡脾脏中lncRNA表达谱的研究。我们的发现强调了p53相关的新型lncRNA803在MDV发病机制中的潜在作用，并为解码涉及非编码RNA的MDV发病机制提供了有价值的见解。
