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Abstract：
The establishment of a stable animal model for intrauterine adhesion (IUA) can significantly enhance research on the pathogenesis and pathological changes of this disease, as well as on the development of innovative therapeutic approaches. In this study, three different modeling methods, including phenol mucilage combined mechanical scraping, ethanol combined mechanical scraping and ethanol modeling alone were designed. The morphological characteristics of the models were evaluated. The underlying mechanisms and fertility capacity of the ethanol modeling group were analyzed and compared to those of the sham surgery group. All three methods resulted in severe intrauterine adhesions, with ethanol being identified as a reliable modeling agent and was subsequently subjected to further evaluation. Immunohistochemistry and RT-PCR results indicated that the ethanol modeling group exhibited an increase in the degree of fibrosis and inflammation, as well as a significant reduction in endometrial thickness, gland number, vascularization, and endometrial receptivity, ultimately resulting in the loss of fertility capacity. The aforementioned findings indicate that the intrauterine perfusion of 95 % ethanol is efficacious in inducing the development of intrauterine adhesions in rats. Given its cost-effectiveness, efficacy, and stability in IUA formation, the use of 95 % ethanol intrauterine perfusion may serve as a novel platform for evaluating innovative anti-adhesion materials and bioengineered therapies.
摘要：
建立稳定的宫腔粘连(IUA)动物模型可显著加强对该病发病机制及病理变化的研究,以及创新治疗方法的发展。在这项研究中,三种不同的建模方法，包括苯酚胶浆联合机械刮擦，设计了乙醇联合机械刮削和乙醇单独建模。对模型的形态特征进行了评价。分析乙醇模型组的潜在机制和生育能力，并与假手术组进行比较。这三种方法都导致了严重的宫腔粘连,乙醇被确定为可靠的模型剂，随后进行了进一步评估。免疫组织化学和RT-PCR结果显示乙醇模型组纤维化和炎症程度增加,以及子宫内膜厚度的显著减少，腺体数,血管化,和子宫内膜容受性，最终导致生育能力的丧失。上述发现表明，子宫内灌注95％乙醇可有效诱导大鼠宫腔粘连的发展。鉴于其成本效益，功效,和IUA形成的稳定性，使用95%乙醇宫内灌注可能成为评估创新抗粘连材料和生物工程疗法的新平台.
