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Abstract：
BACKGROUND: Premature ovarian insufficiency (POI) is a condition that causes secondary amenorrhea owing to ovarian hypofunction at an early stage. Early follicular depletion results in intractable infertility, thereby considerably reducing the quality of life of females. Given the continuum in weakened ovarian function, progressing from incipient ovarian failure (IOF) to transitional ovarian failure and further to POI, it is necessary to develop biomarkers for predicting POI. The oxidative stress states in IOF and POI were comprehensively evaluated via oxidative stress [diacron-reactive oxygen metabolites (d-ROMs)] test and antioxidant capacity [biological antioxidant potential (BAP)].
OBJECTIVE: To explore the possibilities of oxidative stress and antioxidant capacity as biomarkers for the early detection of POI.
METHODS: Females presenting with secondary amenorrhea over 4 mo and a follicle stimulating hormone level of > 40 mIU/mL were categorized into the POI group. Females presenting with a normal menstrual cycle and a follicle stimulating hormone level of > 10.2 mIU/mL were categorized into the IOF group. Healthy females without ovarian hypofunction were categorized into the control group. Among females aged < 40 years who visited our hospital from January 2021 to June 2022, we recruited 11 patients into both POI and IOF groups. For the potential antioxidant capacity, the relative oxidative stress index (BAP/d-ROMs × 100) was calculated, and the oxidative stress defense system was comprehensively evaluated.
RESULTS: d-ROMs were significantly higher in the POI and IOF groups than in the control group, (478.2 ± 58.7 U.CARR, 434.5 ± 60.6 U.CARR, and 341.1 ± 35.1 U.CARR, respectively) (U.CARR is equivalent to 0.08 mg/dL of hydrogen peroxide). However, no significant difference was found between the POI and IOF groups. Regarding BAP, no significant difference was found between the control, IOF, and POI groups (2078.5 ± 157.4 μmol/L, 2116.2 ± 240.2 μmol/L, and 2029.0 ± 186.4 μmol/L, respectively). The oxidative stress index was significantly higher in the POI and IOF groups than in the control group (23.7 ± 3.3, 20.7 ± 3.6, and 16.5 ± 2.1, respectively). However, no significant difference was found between the POI and IOF groups.
CONCLUSIONS: High levels of oxidative stress suggest that evaluating the oxidative stress state may be a useful indicator for the early detection of POI.
OBJECTIVE: To explore the possibilities of oxidative stress and antioxidant capacity as biomarkers for the early detection of POI.
METHODS: Females presenting with secondary amenorrhea over 4 mo and a follicle stimulating hormone level of > 40 mIU/mL were categorized into the POI group. Females presenting with a normal menstrual cycle and a follicle stimulating hormone level of > 10.2 mIU/mL were categorized into the IOF group. Healthy females without ovarian hypofunction were categorized into the control group. Among females aged < 40 years who visited our hospital from January 2021 to June 2022, we recruited 11 patients into both POI and IOF groups. For the potential antioxidant capacity, the relative oxidative stress index (BAP/d-ROMs × 100) was calculated, and the oxidative stress defense system was comprehensively evaluated.
RESULTS: d-ROMs were significantly higher in the POI and IOF groups than in the control group, (478.2 ± 58.7 U.CARR, 434.5 ± 60.6 U.CARR, and 341.1 ± 35.1 U.CARR, respectively) (U.CARR is equivalent to 0.08 mg/dL of hydrogen peroxide). However, no significant difference was found between the POI and IOF groups. Regarding BAP, no significant difference was found between the control, IOF, and POI groups (2078.5 ± 157.4 μmol/L, 2116.2 ± 240.2 μmol/L, and 2029.0 ± 186.4 μmol/L, respectively). The oxidative stress index was significantly higher in the POI and IOF groups than in the control group (23.7 ± 3.3, 20.7 ± 3.6, and 16.5 ± 2.1, respectively). However, no significant difference was found between the POI and IOF groups.
CONCLUSIONS: High levels of oxidative stress suggest that evaluating the oxidative stress state may be a useful indicator for the early detection of POI.
摘要：
背景：过早卵巢功能不全（POI）是由于早期卵巢功能减退而导致继发性闭经的一种疾病。早期卵泡耗竭导致顽固性不孕，从而大大降低了女性的生活质量。鉴于卵巢功能减弱的连续性，从早期卵巢衰竭（IOF）发展到移行性卵巢衰竭，再到POI，有必要开发预测POI的生物标志物。通过氧化应激[涤纶活性氧代谢产物（d-ROM）]测试和抗氧化能力[生物抗氧化潜能（BAP）]全面评估IOF和POI中的氧化应激状态。
目的：探讨氧化应激和抗氧化能力作为POI早期检测生物标志物的可能性。
方法：出现继发性闭经超过4个月和促卵泡激素水平>40mIU/mL的女性被归类为POI组。月经周期正常且卵泡刺激素水平>10.2mIU/mL的女性被归类为IOF组。无卵巢功能减退的健康女性被归类为对照组。在2021年1月至2022年6月来我院就诊的年龄<40岁的女性中，我们招募了11名患者进入POI和IOF组。对于潜在的抗氧化能力，计算相对氧化应激指数(BAP/d-ROM×100)，并对氧化应激防御系统进行了综合评价。
结果：POI和IOF组的d-ROM明显高于对照组，(478.2±58.7U.CARR,434.5±60.6U.CARR,和341.1±35.1U.CARR，分别)(U.CARR相当于0.08mg/dL的过氧化氢)。然而,POI组和IOF组之间无显著差异。关于BAP,对照组之间没有发现显著差异，IOF,和POI组(2078.5±157.4μmol/L，2116.2±240.2μmol/L,2029.0±186.4μmol/L，分别)。POI组和IOF组的氧化应激指数明显高于对照组（分别为23.7±3.3、20.7±3.6和16.5±2.1）。然而,POI组和IOF组之间无显著差异。
结论：高水平的氧化应激表明评估氧化应激状态可能是早期检测POI的有用指标。
目的：探讨氧化应激和抗氧化能力作为POI早期检测生物标志物的可能性。
方法：出现继发性闭经超过4个月和促卵泡激素水平>40mIU/mL的女性被归类为POI组。月经周期正常且卵泡刺激素水平>10.2mIU/mL的女性被归类为IOF组。无卵巢功能减退的健康女性被归类为对照组。在2021年1月至2022年6月来我院就诊的年龄<40岁的女性中，我们招募了11名患者进入POI和IOF组。对于潜在的抗氧化能力，计算相对氧化应激指数(BAP/d-ROM×100)，并对氧化应激防御系统进行了综合评价。
结果：POI和IOF组的d-ROM明显高于对照组，(478.2±58.7U.CARR,434.5±60.6U.CARR,和341.1±35.1U.CARR，分别)(U.CARR相当于0.08mg/dL的过氧化氢)。然而,POI组和IOF组之间无显著差异。关于BAP,对照组之间没有发现显著差异，IOF,和POI组(2078.5±157.4μmol/L，2116.2±240.2μmol/L,2029.0±186.4μmol/L，分别)。POI组和IOF组的氧化应激指数明显高于对照组（分别为23.7±3.3、20.7±3.6和16.5±2.1）。然而,POI组和IOF组之间无显著差异。
结论：高水平的氧化应激表明评估氧化应激状态可能是早期检测POI的有用指标。
