PDF(Pubmed)
Abstract：
Climate change is a global concern for all life on our planet, including humans and plants. Plants\' growth and development are significantly affected by abiotic stresses, including adverse temperature, inadequate or excess water availability, nutrient deficiency, and salinity. The circadian clock is a master regulator of numerous developmental and metabolic processes in plants. In an effort to identify new clock-related genes and outputs through bioinformatic analysis, we have revealed that CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) play a crucial role in regulating a wide range of abiotic stress responses and target ABSCISIC ACID RESPONSIVE ELEMENTS-BINDING FACTOR3 (ABF3), a key transcription factor in the plant hormone Abscisic acid (ABA)-signaling pathway. Specifically, we found that CCA1 and LHY regulate the expression of ABF3 under diel conditions, as well as seed germination under salinity. Conversely, ABF3 controls the expression of core clock genes and orchestrates the circadian period in a stress-responsive manner. ABF3 delivers the stress signal to the central oscillator by binding to the promoter of CCA1 and LHY. Overall, our study uncovers the reciprocal regulation between ABF3 and CCA1/LHY and molecular mechanisms underlying the interaction between the circadian clock and abiotic stress. This finding may aid in developing molecular and genetic solutions for plants to survive and thrive in the face of climate change.
摘要：
气候变化是我们星球上所有生命的全球关切，包括人类和植物。植物的生长和发育受到非生物胁迫的显著影响，包括不利的温度，水供应不足或过量，营养缺乏,和盐度。生物钟是植物中许多发育和代谢过程的主要调节因子。为了通过生物信息学分析识别新的时钟相关基因和输出，我们揭示了CIRCADIAN时钟相关1（CCA1）和晚期下胚轴（LHY）在调节多种非生物应激反应中起着至关重要的作用，并靶向脱落酸响应元素-结合因子3（ABF3），植物激素脱落酸（ABA）信号通路中的关键转录因子。具体来说,我们发现CCA1和LHY在diel条件下调节ABF3的表达，以及盐度下的种子萌发。相反，ABF3控制核心时钟基因的表达，并以应激反应的方式协调昼夜节律。ABF3通过与CCA1和LHY的启动子结合将应激信号传递到中心振荡器。总的来说,我们的研究揭示了ABF3和CCA1/LHY之间的相互调节以及生物钟和非生物胁迫之间相互作用的分子机制.这一发现可能有助于开发分子和遗传解决方案，使植物在面对气候变化时能够生存和茁壮成长。
