UNASSIGNED: miRNA-155 expression by Real Time -Polymerase Chain Reaction (RT-PCR), and vit D, nuclear factor Kappa-light-chain-enhancer of activated B cells (NF-κB), and Tumor necrosis fact of TNF-α) expression by Enzyme Linked Immunosorbent Assay (ELISA) were assessed.
UNASSIGNED: BD patients had a significantly higher relative expression of microRNA-155 (P< 0.001), it was significantly related to vascular manifestations (P< 0.001). Vit D relative expression was significantly low in BD (P< 0.001). There was a significant rise in miRNA-155 in the active group compared to the inactive group (P< 0.001). A significant decrease in vit D levels (IU) was found in inactive and active individuals suffering from BD when compared to controls (P< 0.001). A significant rise was found in vit D levels in inactive BD cases (P< 0.001). A significant positive correlations were found between miRNA-155, NF-κB, TNF-α, and negative correlations with vit D relative expression in BD patients.
UNASSIGNED: miRNA-155 relative expression is higher in BD is significantly related to vascular manifestations. It may have a relationship to disease activity. Vitamin D relative expression is significantly low in BD patients, which can significantly influence immunomodulatory BD therapy. Vitamin D deficiency linked to active BD.
■通过实时聚合酶链反应(RT-PCR)表达miRNA-155,还有VitD,核因子κ-活化B细胞的轻链增强子(NF-κB),通过酶联免疫吸附试验(ELISA)评估TNF-α)表达的肿瘤坏死事实。
■BD患者的microRNA-155的相对表达显著增高(P<0.001),与血管表现显著相关(P<0.001)。BD中VitD的相对表达显著较低(P<0.001)。与非活性组相比,活性组中miRNA-155显著升高(P<0.001)。与对照组相比,在患有BD的非活动和活动个体中发现维生素D水平(IU)显着降低(P<0.001)。在非活动性BD病例中,vitD水平显着升高(P<0.001)。miRNA-155、NF-κB、TNF-α,BD患者vitD相对表达呈负相关。
■miRNA-155的相对表达在BD中较高与血管表现显著相关。它可能与疾病活动有关。维生素D在BD患者中的相对表达明显较低,这可以显着影响免疫调节BD治疗。维生素D缺乏与活跃的BD有关。