PDF(Pubmed)
Abstract：
UNASSIGNED: Vitamin D (vit D) controls inflammation and immunity. In Behçet\'s disease (BD), microRNA-155 is recognized as a significant immune response regulator. We aimed to investigate the role of vit D on immunomodulation and downregulation of inflammatory pathways associated with BD and detect the role of miRNA-155 in BD.
UNASSIGNED: miRNA-155 expression by Real Time -Polymerase Chain Reaction (RT-PCR), and vit D, nuclear factor Kappa-light-chain-enhancer of activated B cells (NF-κB), and Tumor necrosis fact of TNF-α) expression by Enzyme Linked Immunosorbent Assay (ELISA) were assessed.
UNASSIGNED: BD patients had a significantly higher relative expression of microRNA-155 (P< 0.001), it was significantly related to vascular manifestations (P< 0.001). Vit D relative expression was significantly low in BD (P< 0.001). There was a significant rise in miRNA-155 in the active group compared to the inactive group (P< 0.001). A significant decrease in vit D levels (IU) was found in inactive and active individuals suffering from BD when compared to controls (P< 0.001). A significant rise was found in vit D levels in inactive BD cases (P< 0.001). A significant positive correlations were found between miRNA-155, NF-κB, TNF-α, and negative correlations with vit D relative expression in BD patients.
UNASSIGNED: miRNA-155 relative expression is higher in BD is significantly related to vascular manifestations. It may have a relationship to disease activity. Vitamin D relative expression is significantly low in BD patients, which can significantly influence immunomodulatory BD therapy. Vitamin D deficiency linked to active BD.
UNASSIGNED: miRNA-155 expression by Real Time -Polymerase Chain Reaction (RT-PCR), and vit D, nuclear factor Kappa-light-chain-enhancer of activated B cells (NF-κB), and Tumor necrosis fact of TNF-α) expression by Enzyme Linked Immunosorbent Assay (ELISA) were assessed.
UNASSIGNED: BD patients had a significantly higher relative expression of microRNA-155 (P< 0.001), it was significantly related to vascular manifestations (P< 0.001). Vit D relative expression was significantly low in BD (P< 0.001). There was a significant rise in miRNA-155 in the active group compared to the inactive group (P< 0.001). A significant decrease in vit D levels (IU) was found in inactive and active individuals suffering from BD when compared to controls (P< 0.001). A significant rise was found in vit D levels in inactive BD cases (P< 0.001). A significant positive correlations were found between miRNA-155, NF-κB, TNF-α, and negative correlations with vit D relative expression in BD patients.
UNASSIGNED: miRNA-155 relative expression is higher in BD is significantly related to vascular manifestations. It may have a relationship to disease activity. Vitamin D relative expression is significantly low in BD patients, which can significantly influence immunomodulatory BD therapy. Vitamin D deficiency linked to active BD.
摘要：
■维生素D（维生素D）控制炎症和免疫力。在Behçet病(BD)中，microRNA-155被认为是一种重要的免疫应答调节因子。我们旨在研究vitD在与BD相关的免疫调节和炎症通路下调中的作用，并检测miRNA-155在BD中的作用。
■通过实时聚合酶链反应（RT-PCR）表达miRNA-155，还有VitD,核因子κ-活化B细胞的轻链增强子（NF-κB），通过酶联免疫吸附试验（ELISA）评估TNF-α）表达的肿瘤坏死事实。
■BD患者的microRNA-155的相对表达显著增高(P<0.001),与血管表现显著相关(P<0.001)。BD中VitD的相对表达显著较低(P<0.001)。与非活性组相比,活性组中miRNA-155显著升高(P<0.001)。与对照组相比，在患有BD的非活动和活动个体中发现维生素D水平（IU）显着降低（P<0.001）。在非活动性BD病例中，vitD水平显着升高（P<0.001）。miRNA-155、NF-κB、TNF-α,BD患者vitD相对表达呈负相关。
■miRNA-155的相对表达在BD中较高与血管表现显著相关。它可能与疾病活动有关。维生素D在BD患者中的相对表达明显较低，这可以显着影响免疫调节BD治疗。维生素D缺乏与活跃的BD有关。
■通过实时聚合酶链反应（RT-PCR）表达miRNA-155，还有VitD,核因子κ-活化B细胞的轻链增强子（NF-κB），通过酶联免疫吸附试验（ELISA）评估TNF-α）表达的肿瘤坏死事实。
■BD患者的microRNA-155的相对表达显著增高(P<0.001),与血管表现显著相关(P<0.001)。BD中VitD的相对表达显著较低(P<0.001)。与非活性组相比,活性组中miRNA-155显著升高(P<0.001)。与对照组相比，在患有BD的非活动和活动个体中发现维生素D水平（IU）显着降低（P<0.001）。在非活动性BD病例中，vitD水平显着升高（P<0.001）。miRNA-155、NF-κB、TNF-α,BD患者vitD相对表达呈负相关。
■miRNA-155的相对表达在BD中较高与血管表现显著相关。它可能与疾病活动有关。维生素D在BD患者中的相对表达明显较低，这可以显着影响免疫调节BD治疗。维生素D缺乏与活跃的BD有关。
