Abstract：
Hantaviruses, are rodent-borne viruses found worldwide that are transmitted to humans through inhalation of contaminated excreta. They can cause a renal or a pulmonary syndrome, depending on the virus, and no effective treatment is currently available for either of these diseases. Hantaviral particles are covered by a protein lattice composed of two glycoproteins (Gn and Gc) that mediate adsorption to target cells and fusion with endosomal membranes, making them prime targets for neutralizing antibodies. Here we present the methodology to produce soluble recombinant glycoproteins in different conformations, either alone or as a stabilized Gn/Gc complex, using stably transfected Drosophila S2 cells.
摘要：
汉坦病毒,是全世界发现的啮齿动物传播的病毒，通过吸入受污染的排泄物传播给人类。它们会导致肾或肺综合征，取决于病毒，目前对这两种疾病都没有有效的治疗方法。汉坦病毒颗粒被由两种糖蛋白（Gn和Gc）组成的蛋白质晶格覆盖，这些糖蛋白介导对靶细胞的吸附并与内体膜融合，使它们成为中和抗体的主要目标。在这里，我们提出了生产不同构象的可溶性重组糖蛋白的方法，单独或作为稳定的Gn/Gc复合物，使用稳定转染的果蝇S2细胞。
