PDF(Pubmed)
Abstract：
Achromobacter spp. are opportunistic pathogens of environmental origin increasingly isolated in patients with underlying conditions like cystic fibrosis (CF). Despite recent advances, their virulence factors remain incompletely studied, and siderophore production has not yet been investigated in this genus. The aim of this study was to evaluate the production of siderophores in a large collection of Achromobacter spp. and evaluate the variability according to the origin of the strain and species. A total of 163 strains were studied, including 128 clinical strains (CF and non-CF patients) and 35 strains of environmental origin. Siderophores were quantified by the liquid chrome azurol-sulphonate assay. Species were identified by nrdA gene-based phylogeny. Strains were assigned to 20 species, with Achromobacter xylosoxidans being the most represented (51.5% of strains). Siderophore production was observed in 72.4% of the strains, with amounts ranging from 10.1% to 90% siderophore units. A significantly higher prevalence of siderophore-producing strains and greater production of siderophores were observed for clinical strains compared with strains of environmental origin. Highly variable observations were made according to species: A. xylosoxidans presented unique characteristics (one of the highest prevalence of producing strains and highest amounts produced, particularly by CF strains). Siderophores are important factors for bacterial growth commonly produced by members of the Achromobacter genus. The significance of the observations made during this study must be further investigated. Indeed, the differences observed according to species and the origin of strains suggest that siderophores may represent important determinants of the pathophysiology of Achromobacter spp. infections and also contribute to the particular epidemiological success of A. xylosoxidans in human infections.
OBJECTIVE: Achromobacter spp. are recognized as emerging opportunistic pathogens in humans with various underlying diseases, including cystic fibrosis (CF). Although their pathophysiological traits are increasingly studied, their virulence factors remain incompletely described. Particularly, siderophores that represent important factors of bacterial growth have not yet been studied in this genus. A population-based study was performed to explore the ability of members of the Achromobacter genus to produce siderophores, both overall and in relevant subgroups (Achromobacter species; strain origin, either clinical-from CF or non-CF patients-or environmental). This study provides original data showing that siderophore production is a common trait of Achromobacter strains, particularly observed among clinical strains. The major species, Achromobacter xylosoxidans, encompassed both one of the highest prevalence of siderophore-producing strains and strains producing the largest amounts of siderophores, particularly observed for CF strains. These observations may represent additional advantages accounting for the epidemiological success of this species.
OBJECTIVE: Achromobacter spp. are recognized as emerging opportunistic pathogens in humans with various underlying diseases, including cystic fibrosis (CF). Although their pathophysiological traits are increasingly studied, their virulence factors remain incompletely described. Particularly, siderophores that represent important factors of bacterial growth have not yet been studied in this genus. A population-based study was performed to explore the ability of members of the Achromobacter genus to produce siderophores, both overall and in relevant subgroups (Achromobacter species; strain origin, either clinical-from CF or non-CF patients-or environmental). This study provides original data showing that siderophore production is a common trait of Achromobacter strains, particularly observed among clinical strains. The major species, Achromobacter xylosoxidans, encompassed both one of the highest prevalence of siderophore-producing strains and strains producing the largest amounts of siderophores, particularly observed for CF strains. These observations may represent additional advantages accounting for the epidemiological success of this species.
摘要：
无色杆菌属。在患有囊性纤维化（CF）等潜在疾病的患者中，环境来源的机会病原体越来越多。尽管最近取得了进展，它们的毒力因子仍未得到充分研究，和铁载体的生产尚未在该属中进行研究。这项研究的目的是评估大量无色杆菌属中铁载体的生产。并根据菌株和物种的起源评估变异性。共研究了163个菌株，包括128个临床菌株（CF和非CF患者）和35个环境来源的菌株。通过液体铬天青-磺酸盐测定法定量铁载体。通过基于nrdA基因的系统发育鉴定物种。菌株被分配给20个物种，以木氧歧化杆菌为代表最多（占菌株的51.5%）。在72.4％的菌株中观察到铁载体的产生，含量范围为10.1％至90％的铁载体单位。与环境来源的菌株相比，临床菌株的产铁载体菌株的患病率明显更高，而铁载体的产量更高。根据物种进行了高度可变的观察：A.xylosoxidans具有独特的特征（生产菌株的患病率最高，产量最高，特别是CF菌株)。铁载体是通常由无色杆菌属成员产生的细菌生长的重要因素。在这项研究中观察到的意义必须进一步研究。的确,根据物种和菌株起源观察到的差异表明，铁载体可能代表无色杆菌属病理生理学的重要决定因素。感染，并且还有助于木酮氧化菌在人类感染中的特殊流行病学成功。重要细菌。在患有各种潜在疾病的人类中被认为是新兴的机会病原体，包括囊性纤维化（CF）。尽管对它们的病理生理特性的研究越来越多,其毒力因子仍未完全描述。特别是,代表细菌生长重要因素的铁载体尚未在该属中进行研究。进行了一项基于人群的研究，以探索无色杆菌属成员产生铁载体的能力，整体和相关亚组(无色杆菌属；菌株起源，临床-来自CF或非CF患者-或环境)。这项研究提供了原始数据，表明铁载体的产生是嗜铬杆菌菌株的共同特征，特别是在临床菌株中观察到。主要物种,木氧嗜酸性杆菌，既包括流行率最高的产生铁载体的菌株之一，也包括产生最大量铁载体的菌株，特别是CF菌株。这些观察结果可能代表了该物种流行病学成功的其他优势。
