PDF(Pubmed)
Abstract：
UNASSIGNED: Trehalose is vital for plant metabolism, growth, and stress resilience, relying on Trehalose-6-phosphate synthase (TPS) and Trehalose-6-phosphate phosphatase (TPP) genes. Research on these genes in cultivated peanuts (Arachis hypogaea) is limited.
UNASSIGNED: This study employed bioinformatics to identify and analyze AhTPS and AhTPP genes in cultivated peanuts, with subsequent experimental validation of AhTPS9\'s role in cold tolerance.
UNASSIGNED: In the cultivated peanut genome, a total of 16 AhTPS and 17 AhTPP genes were identified. AhTPS and AhTPP genes were observed in phylogenetic analysis, closely related to wild diploid peanuts, respectively. The evolutionary patterns of AhTPS and AhTPP genes were predominantly characterized by gene segmental duplication events and robust purifying selection. A variety of hormone-responsive and stress-related cis-elements were unveiled in our analysis of cis-regulatory elements. Distinct expression patterns of AhTPS and AhTPP genes across different peanut tissues, developmental stages, and treatments were revealed, suggesting potential roles in growth, development, and stress responses. Under low-temperature stress, qPCR results showcased upregulation in AhTPS genes (AhTPS2-5, AhTPS9-12, AhTPS14, AhTPS15) and AhTPP genes (AhTPP1, AhTPP6, AhTPP11, AhTPP13). Furthermore, AhTPS9, exhibiting the most significant expression difference under cold stress, was obviously induced by cold stress in cultivated peanut, and AhTPS9-overexpression improved the cold tolerance of Arabidopsis by protect the photosynthetic system of plants, and regulates sugar-related metabolites and genes.
UNASSIGNED: This comprehensive study lays the groundwork for understanding the roles of AhTPS and AhTPP gene families in trehalose regulation within cultivated peanuts and provides valuable insights into the mechanisms related to cold stress tolerance.
UNASSIGNED: This study employed bioinformatics to identify and analyze AhTPS and AhTPP genes in cultivated peanuts, with subsequent experimental validation of AhTPS9\'s role in cold tolerance.
UNASSIGNED: In the cultivated peanut genome, a total of 16 AhTPS and 17 AhTPP genes were identified. AhTPS and AhTPP genes were observed in phylogenetic analysis, closely related to wild diploid peanuts, respectively. The evolutionary patterns of AhTPS and AhTPP genes were predominantly characterized by gene segmental duplication events and robust purifying selection. A variety of hormone-responsive and stress-related cis-elements were unveiled in our analysis of cis-regulatory elements. Distinct expression patterns of AhTPS and AhTPP genes across different peanut tissues, developmental stages, and treatments were revealed, suggesting potential roles in growth, development, and stress responses. Under low-temperature stress, qPCR results showcased upregulation in AhTPS genes (AhTPS2-5, AhTPS9-12, AhTPS14, AhTPS15) and AhTPP genes (AhTPP1, AhTPP6, AhTPP11, AhTPP13). Furthermore, AhTPS9, exhibiting the most significant expression difference under cold stress, was obviously induced by cold stress in cultivated peanut, and AhTPS9-overexpression improved the cold tolerance of Arabidopsis by protect the photosynthetic system of plants, and regulates sugar-related metabolites and genes.
UNASSIGNED: This comprehensive study lays the groundwork for understanding the roles of AhTPS and AhTPP gene families in trehalose regulation within cultivated peanuts and provides valuable insights into the mechanisms related to cold stress tolerance.
摘要：
■海藻糖对植物代谢至关重要，增长,和压力弹性，依赖于海藻糖-6-磷酸合酶（TPS）和海藻糖-6-磷酸磷酸酶（TPP）基因。在栽培花生（Arachishypogaea）中对这些基因的研究有限。
■这项研究采用生物信息学来鉴定和分析栽培花生中的AhTPS和AhTPP基因，随后实验验证了AhTPS9在耐寒性中的作用。
■在栽培花生基因组中，共鉴定出16个AhTPS和17个AhTPP基因。在系统发育分析中观察到AhTPS和AhTPP基因，与野生二倍体花生密切相关，分别。AhTPS和AhTPP基因的进化模式主要以基因片段重复事件和强大的纯化选择为特征。在我们对顺式调节元件的分析中，揭示了各种激素反应性和与压力相关的顺式元件。AhTPS和AhTPP基因在不同花生组织中的不同表达模式，发育阶段,并揭示了治疗方法，暗示增长中的潜在角色，发展,和应激反应。在低温胁迫下,qPCR结果显示AhTPS基因(AhTPS2-5、AhTPS9-12、AhTPS14、AhTPS15)和AhTPP基因(AhTPP1、AhTPP6、AhTPP11、AhTPP13)表达上调。此外，AhTPS9在冷胁迫下表现出最显著的表达差异，在栽培花生中明显受到冷胁迫的诱导，AhTPS9过表达通过保护植物的光合系统提高了拟南芥的耐寒性，并调节与糖相关的代谢产物和基因。
■这项全面的研究为理解AhTPS和AhTPP基因家族在栽培花生内海藻糖调节中的作用奠定了基础，并为与冷胁迫耐受性相关的机制提供了有价值的见解。
■这项研究采用生物信息学来鉴定和分析栽培花生中的AhTPS和AhTPP基因，随后实验验证了AhTPS9在耐寒性中的作用。
■在栽培花生基因组中，共鉴定出16个AhTPS和17个AhTPP基因。在系统发育分析中观察到AhTPS和AhTPP基因，与野生二倍体花生密切相关，分别。AhTPS和AhTPP基因的进化模式主要以基因片段重复事件和强大的纯化选择为特征。在我们对顺式调节元件的分析中，揭示了各种激素反应性和与压力相关的顺式元件。AhTPS和AhTPP基因在不同花生组织中的不同表达模式，发育阶段,并揭示了治疗方法，暗示增长中的潜在角色，发展,和应激反应。在低温胁迫下,qPCR结果显示AhTPS基因(AhTPS2-5、AhTPS9-12、AhTPS14、AhTPS15)和AhTPP基因(AhTPP1、AhTPP6、AhTPP11、AhTPP13)表达上调。此外，AhTPS9在冷胁迫下表现出最显著的表达差异，在栽培花生中明显受到冷胁迫的诱导，AhTPS9过表达通过保护植物的光合系统提高了拟南芥的耐寒性，并调节与糖相关的代谢产物和基因。
■这项全面的研究为理解AhTPS和AhTPP基因家族在栽培花生内海藻糖调节中的作用奠定了基础，并为与冷胁迫耐受性相关的机制提供了有价值的见解。
