PDF(Pubmed)
Abstract：
BACKGROUND: Infections of cats with Echinococcus granulosus is uncommon because the cat is not part of the parasite life cycle that a carnivorous and another herbivore represent. Nevertheless, it occurs incidentally when eating food or drinking water contaminated with the worm\'s larva, especially with the presence of the definitive host (dogs), in this case, the infections are concentrated in stray or outside cats. For this reason, this study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection.
OBJECTIVE: This study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection.
METHODS: Four of the 37 cats that had died in different accidents developed cystic echinococcosis (CE). The cytochrome c oxidase subunit I (COX1) gene was initially amplified and sequenced to determine if these cysts belonged to E. granulosus, in beginning. The DNA fragments resulting from sequencing were then compared and aligned with other sequences using the Gene Bank database. Finally, a phylogenetic tree was drawn according to the sequence data obtained from cox1 genes sequencing, and the MEGA 7.0 phylogenetic analysis program was utilized.
RESULTS: Four different sequences were deposited in the Gen Bank with accession numbers (ON795961 to ON795964), all of which belong to the G1 genotype. Approximately 84% and 100% of these sequences aligned with G1 (AB622277.1) and G1 (MG722980.1), respectively.
CONCLUSIONS: G1 is the dominant genotype that causes cat infections, even though the cat\'s EC infection was incidental.
OBJECTIVE: This study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection.
METHODS: Four of the 37 cats that had died in different accidents developed cystic echinococcosis (CE). The cytochrome c oxidase subunit I (COX1) gene was initially amplified and sequenced to determine if these cysts belonged to E. granulosus, in beginning. The DNA fragments resulting from sequencing were then compared and aligned with other sequences using the Gene Bank database. Finally, a phylogenetic tree was drawn according to the sequence data obtained from cox1 genes sequencing, and the MEGA 7.0 phylogenetic analysis program was utilized.
RESULTS: Four different sequences were deposited in the Gen Bank with accession numbers (ON795961 to ON795964), all of which belong to the G1 genotype. Approximately 84% and 100% of these sequences aligned with G1 (AB622277.1) and G1 (MG722980.1), respectively.
CONCLUSIONS: G1 is the dominant genotype that causes cat infections, even though the cat\'s EC infection was incidental.
摘要：
背景：细粒棘球蚴感染的猫并不常见，因为猫不是食肉和另一种食草动物所代表的寄生虫生命周期的一部分。然而，它偶然发生在食用被蠕虫幼虫污染的食物或饮用水时，特别是在确定的宿主（狗）的存在下，在这种情况下,感染集中在流浪猫或外面的猫。出于这个原因,这项研究检查了猫感染细粒大肠杆菌的可能性，并诊断了这种感染的常见基因型。
目的：本研究检测了猫感染细粒大肠杆菌的可能性，并诊断了这种感染的常见基因型。
方法：在不同事故中死亡的37只猫中，有4只发生了囊性包虫病（CE）。最初扩增细胞色素c氧化酶亚基I（COX1）基因并进行测序，以确定这些囊肿是否属于E.granulosus，在开始。然后使用GeneBank数据库将由测序产生的DNA片段与其他序列进行比较和比对。最后,根据cox1基因测序获得的序列数据绘制系统发育树，并使用了MEGA7.0系统发育分析程序。
结果：四种不同的序列被存入GenBank，登录号（ON795961至ON795964），所有这些都属于G1基因型。这些序列中大约84%和100%与G1(AB622277.1)和G1(MG722980.1)对齐，分别。
结论：G1是导致猫感染的主要基因型，即使猫的EC感染是偶然的。
目的：本研究检测了猫感染细粒大肠杆菌的可能性，并诊断了这种感染的常见基因型。
方法：在不同事故中死亡的37只猫中，有4只发生了囊性包虫病（CE）。最初扩增细胞色素c氧化酶亚基I（COX1）基因并进行测序，以确定这些囊肿是否属于E.granulosus，在开始。然后使用GeneBank数据库将由测序产生的DNA片段与其他序列进行比较和比对。最后,根据cox1基因测序获得的序列数据绘制系统发育树，并使用了MEGA7.0系统发育分析程序。
结果：四种不同的序列被存入GenBank，登录号（ON795961至ON795964），所有这些都属于G1基因型。这些序列中大约84%和100%与G1(AB622277.1)和G1(MG722980.1)对齐，分别。
结论：G1是导致猫感染的主要基因型，即使猫的EC感染是偶然的。
