Abstract：
Translationally controlled tumor protein (TCTP) is a highly conserved multifunctional protein, which participates in many important physiological processes. Recently, the roles of TCTP in cell proliferation and apoptosis, especially its close relationship with various tumors, have attracted widespread attention. In this study, we found that the protein level of TCTP was significantly reduced in acute promyelocytic leukemia cell line NB4 transfected with retinoic acid-induced gene G (RIG-G). The RIG-G was found in our previous work as a key mediator of anti-proliferative activity in retinoid/interferon-related pathways. Here, we tried to further explore the function of TCTP in the development of acute myeloid leukemia (AML) from different levels. Our results showed that inhibiting TCTP expression could attenuate AML cells proliferation and induce apoptosis both in AML cell lines and in xenograft of NOD-SCID mice. In addition, either compared with patients in complete remission or non-leukemia patients, we detected that the expression of TCTP was generally high in the fresh bone marrow of AML patients, suggesting that there was a certain correlation between TCTP and AML disease progression. Taken together, our study revealed the role of TCTP in AML development, and provided a potential target for AML treatment.
摘要：
翻译控制肿瘤蛋白(TCTP)是一种高度保守的多功能蛋白,参与许多重要的生理过程。最近,TCTP在细胞增殖和凋亡中的作用,特别是它与各种肿瘤的密切关系，引起了广泛的关注。在这项研究中,我们发现在转染维甲酸诱导基因G(RIG-G)的急性早幼粒细胞白血病细胞株NB4中,TCTP蛋白水平显著降低.在我们之前的工作中发现RIG-G是类维生素A/干扰素相关途径中抗增殖活性的关键介质。这里,我们试图从不同层面进一步探讨TCTP在急性髓系白血病（AML）发生发展中的作用。我们的结果表明，抑制TCTP表达可以减弱AML细胞系和NOD-SCID小鼠异种移植物中的AML细胞增殖并诱导凋亡。此外,与完全缓解患者或非白血病患者相比，我们检测到TCTP在AML患者的新鲜骨髓中普遍高表达,提示TCTP与AML疾病进展有一定的相关性。一起来看,我们的研究揭示了TCTP在AML发展中的作用，并为AML治疗提供了潜在的靶点。
