PDF(Pubmed)
Abstract：
Halari donkey breed is one of the indigenous breeds of India and its population is rapidly decreasing. The Jenny milk is more similar to human milk, exhibits a wide range of probiotic diversity and hypo-allergenicity, especially among infants suffering from cow and buffalo milk protein allergy. Some studies indicated low levels of κ-casein fraction of casein protein in donkey milk. The k-casein gene was not amplified from the DNA derived from the milk somatic cells of Halari donkeys. The Halari donkey milk has low somatic cells count. We report the first isolation of DNA from milk somatic cells of Halari donkeys with subsequent characterization of k-casein gene. Through our work, we showed that the milk somatic cells can be used as a non-invasive source for DNA isolation towards molecular studies. It also proved the presence of k-casein gene in Halari donkey milk by its amplification from isolated DNA.
摘要：
Halari驴品种是印度的土著品种之一，其人口正在迅速减少。珍妮的牛奶更像人奶，具有广泛的益生菌多样性和低致敏性，尤其是患有牛和水牛奶蛋白过敏的婴儿。一些研究表明，驴乳中酪蛋白的κ-酪蛋白含量较低。从Halari驴的乳体细胞衍生的DNA中没有扩增k-酪蛋白基因。Halari驴奶的体细胞数量较低。我们报告了从Halari驴的牛奶体细胞中首次分离DNA，随后对k-酪蛋白基因进行了表征。通过我们的工作,我们表明，牛奶体细胞可以用作分子研究中DNA分离的非侵入性来源。通过从分离的DNA中扩增，还证明了Halari驴乳中存在k-酪蛋白基因。
