PDF(Pubmed)
Abstract：
UNASSIGNED: The aim of the study was to compare the ameliorating effects of thymoquinone at various dosages on cisplatin-induced renal toxicity, and to investigate its effects on cisplatin-induced nephrocyte apoptosis via the mitochondrial pathway in a rat model.
UNASSIGNED: A rat model of cisplatin-induced renal damage was established, with thymoquinone treatment groups (receiving 1, 3, 5, 10, or 20 mg/kg of thymoquinone). We determined serum creatinine (Cr) and blood urea nitrogen (BUN), measured the expression of the anti-apoptotic protein Bcl-2, the pro-apoptotic protein Bax, caspase-3, kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) in renal tissue. Additionally, we observed pathological changes in renal tissue and performed paller score for renal tubule injury.
UNASSIGNED: Relative to the control, the cisplatin group exhibited significantly elevated Bax, caspase-3, NGAL and KIM-1 expression, elevated serum Cr and BUN concentrations and significantly reduced Bcl-2 expression (P < 0.05). Histopathological examination of cisplatin-treated group revealed vacuolar degeneration, tubular epithelial cell swelling, and an absence of brush margins on renal tubules. Paller score was significantly elevated in the cisplatin group relative to the normal control group. Thymoquinone dose-dependently ameliorated these effects.
UNASSIGNED: Thymoquinone at 1-20 mg/kg improved cisplatin-induced renal dysfunction in rats. This protective effect is related to the inhibition of mitochondria-mediated apoptosis.
UNASSIGNED: A rat model of cisplatin-induced renal damage was established, with thymoquinone treatment groups (receiving 1, 3, 5, 10, or 20 mg/kg of thymoquinone). We determined serum creatinine (Cr) and blood urea nitrogen (BUN), measured the expression of the anti-apoptotic protein Bcl-2, the pro-apoptotic protein Bax, caspase-3, kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) in renal tissue. Additionally, we observed pathological changes in renal tissue and performed paller score for renal tubule injury.
UNASSIGNED: Relative to the control, the cisplatin group exhibited significantly elevated Bax, caspase-3, NGAL and KIM-1 expression, elevated serum Cr and BUN concentrations and significantly reduced Bcl-2 expression (P < 0.05). Histopathological examination of cisplatin-treated group revealed vacuolar degeneration, tubular epithelial cell swelling, and an absence of brush margins on renal tubules. Paller score was significantly elevated in the cisplatin group relative to the normal control group. Thymoquinone dose-dependently ameliorated these effects.
UNASSIGNED: Thymoquinone at 1-20 mg/kg improved cisplatin-induced renal dysfunction in rats. This protective effect is related to the inhibition of mitochondria-mediated apoptosis.
摘要：
■本研究的目的是比较不同剂量的百里香醌对顺铂诱导的肾毒性的改善作用，并探讨其通过线粒体途径对顺铂诱导的大鼠肾细胞凋亡的影响。
■建立顺铂肾损害大鼠模型,百里香醌治疗组(接受1、3、5、10或20mg/kg百里香醌)。我们测定了血清肌酐（Cr）和血尿素氮（BUN），检测抗凋亡蛋白Bcl-2、促凋亡蛋白Bax、caspase-3，肾损伤分子-1（KIM-1）和肾组织中的中性粒细胞明胶酶相关脂质运载蛋白（NGAL）。此外,观察肾组织病理变化，并对肾小管损伤进行paller评分。
■相对于对照，顺铂组出现显著升高的Bax，caspase-3、NGAL和KIM-1表达，血清Cr和BUN浓度升高,Bcl-2表达显著降低(P<0.05)。顺铂治疗组的组织病理学检查显示空泡变性，肾小管上皮细胞肿胀,肾小管上没有刷缘。与正常对照组相比，顺铂组的Paller评分显着升高。胸腺醌剂量依赖性地改善了这些作用。
■1-20mg/kg的胸腺醌改善了顺铂诱导的大鼠肾功能障碍。这种保护作用与抑制线粒体介导的细胞凋亡有关。
■建立顺铂肾损害大鼠模型,百里香醌治疗组(接受1、3、5、10或20mg/kg百里香醌)。我们测定了血清肌酐（Cr）和血尿素氮（BUN），检测抗凋亡蛋白Bcl-2、促凋亡蛋白Bax、caspase-3，肾损伤分子-1（KIM-1）和肾组织中的中性粒细胞明胶酶相关脂质运载蛋白（NGAL）。此外,观察肾组织病理变化，并对肾小管损伤进行paller评分。
■相对于对照，顺铂组出现显著升高的Bax，caspase-3、NGAL和KIM-1表达，血清Cr和BUN浓度升高,Bcl-2表达显著降低(P<0.05)。顺铂治疗组的组织病理学检查显示空泡变性，肾小管上皮细胞肿胀,肾小管上没有刷缘。与正常对照组相比，顺铂组的Paller评分显着升高。胸腺醌剂量依赖性地改善了这些作用。
■1-20mg/kg的胸腺醌改善了顺铂诱导的大鼠肾功能障碍。这种保护作用与抑制线粒体介导的细胞凋亡有关。
