PDF(Pubmed)
Abstract：
UNASSIGNED: Odontogenic, non-inflammatory maxillofacial cysts and tumours vary greatly in their ability to grow and cause local tissue destruction. Despite their common embryologic origin, the biologic mechanisms responsible for this diverse array of clinical behaviour are largely unknown. Unfortunately, even with accurate tissue diagnosis and appropriate surgical management, these tumours have relatively high recurrence rates. While this may be related to surgical technique, it may also be due to intrinsic tumour biology. SOX2 is differentially expressed in odontogenic cysts and tumours, which has an impact over patient prognosis. This could be related to their diverse cells of origin or stages of histogenesis. SOX2 is expressed in OKC and ameloblastoma, and in this study, we look forward to find altered levels and intensity of SOX2 in the above-mentioned lesions.
UNASSIGNED: To profile the expression of SOX2 in odontogenic keratocyst (OKC) and ameloblastomaTo compare the intensity of these lesions, analyse their intrinsic feature and predict their recurrence.
UNASSIGNED: Histopathologically diagnosed cases of OKC and ameloblastoma will be selected (n = 40). Paraffin-embedded, formalin-fixed sections of these lesions will be stained for SOX2 marker using a standard immunohistochemical technique. Positive control will be taken as oral squamous cell carcinoma and negative control will be taken as normal oral mucosa.
UNASSIGNED: A comparison between the stained cell types in odontogenic keratocyst and ameloblastoma revealed statistically significant differences. The immunoreactivity scores of SOX2 were analysed in both groups. The results indicated that 45% of OKC cases exhibited strongly positive reactivity, while 65% of ameloblastoma cases were negative. Statistical analysis demonstrated highly significant differences in the frequency of SOX2 expression between the two groups, with a higher frequency of negative expression in ameloblastoma.
UNASSIGNED: Stem cell markers have been observed in these lesions, suggesting the acquisition of stem-like properties by tumour cells, which can affect patient prognosis. Specifically, the marker SOX2 shows differential expression in odontogenic cysts and tumours. High expression of SOX2 in OKC indicates the presence of stem cells with significant self-renewal and proliferative properties, potentially signifying neoplastic behaviour. In contrast, weak or absent expression of SOX2 in ameloblastoma suggests different molecular pathways involved in its neoplastic behaviour.
UNASSIGNED: To profile the expression of SOX2 in odontogenic keratocyst (OKC) and ameloblastomaTo compare the intensity of these lesions, analyse their intrinsic feature and predict their recurrence.
UNASSIGNED: Histopathologically diagnosed cases of OKC and ameloblastoma will be selected (n = 40). Paraffin-embedded, formalin-fixed sections of these lesions will be stained for SOX2 marker using a standard immunohistochemical technique. Positive control will be taken as oral squamous cell carcinoma and negative control will be taken as normal oral mucosa.
UNASSIGNED: A comparison between the stained cell types in odontogenic keratocyst and ameloblastoma revealed statistically significant differences. The immunoreactivity scores of SOX2 were analysed in both groups. The results indicated that 45% of OKC cases exhibited strongly positive reactivity, while 65% of ameloblastoma cases were negative. Statistical analysis demonstrated highly significant differences in the frequency of SOX2 expression between the two groups, with a higher frequency of negative expression in ameloblastoma.
UNASSIGNED: Stem cell markers have been observed in these lesions, suggesting the acquisition of stem-like properties by tumour cells, which can affect patient prognosis. Specifically, the marker SOX2 shows differential expression in odontogenic cysts and tumours. High expression of SOX2 in OKC indicates the presence of stem cells with significant self-renewal and proliferative properties, potentially signifying neoplastic behaviour. In contrast, weak or absent expression of SOX2 in ameloblastoma suggests different molecular pathways involved in its neoplastic behaviour.
摘要：
■牙源性，非炎性颌面部囊肿和肿瘤的生长和引起局部组织破坏的能力差异很大。尽管它们有共同的胚胎起源,导致这种不同临床行为的生物学机制在很大程度上是未知的.不幸的是,即使有准确的组织诊断和适当的手术管理，这些肿瘤的复发率相对较高。虽然这可能与手术技术有关，这也可能是由于内在的肿瘤生物学。SOX2在牙源性囊肿和肿瘤中差异表达，这对患者预后有影响。这可能与它们不同的起源细胞或组织发生阶段有关。SOX2在OKC和成釉细胞瘤中表达，在这项研究中，我们期待在上述病变中发现SOX2水平和强度的改变.
■分析SOX2在牙源性角化囊肿（OKC）和成釉细胞瘤中的表达，比较这些病变的强度，分析其内在特征并预测其复发。
■将选择经组织病理学诊断的OKC和成釉细胞瘤病例(n=40)。石蜡包埋，将使用标准免疫组织化学技术对这些损伤的福尔马林固定切片进行SOX2标记染色。阳性对照将被视为口腔鳞状细胞癌,阴性对照将被视为正常口腔粘膜。
■牙源性角化囊肿和成釉细胞瘤中染色细胞类型之间的比较显示出统计学上的显着差异。分析两组的SOX2免疫反应性评分。结果表明，45%的OKC病例表现出强烈的阳性反应，而65%的成釉细胞瘤病例为阴性。统计学分析显示两组间SOX2表达频率有高度显著性差异,成釉细胞瘤中阴性表达频率较高。
■在这些病变中已观察到干细胞标志物，表明肿瘤细胞获得了干细胞样的特性，这会影响患者的预后。具体来说,标记SOX2在牙源性囊肿和肿瘤中显示差异表达。SOX2在OKC中的高表达表明存在具有显著自我更新和增殖特性的干细胞,潜在的肿瘤行为。相比之下,成釉细胞瘤中SOX2的弱表达或缺失表明其肿瘤行为涉及不同的分子途径。
■分析SOX2在牙源性角化囊肿（OKC）和成釉细胞瘤中的表达，比较这些病变的强度，分析其内在特征并预测其复发。
■将选择经组织病理学诊断的OKC和成釉细胞瘤病例(n=40)。石蜡包埋，将使用标准免疫组织化学技术对这些损伤的福尔马林固定切片进行SOX2标记染色。阳性对照将被视为口腔鳞状细胞癌,阴性对照将被视为正常口腔粘膜。
■牙源性角化囊肿和成釉细胞瘤中染色细胞类型之间的比较显示出统计学上的显着差异。分析两组的SOX2免疫反应性评分。结果表明，45%的OKC病例表现出强烈的阳性反应，而65%的成釉细胞瘤病例为阴性。统计学分析显示两组间SOX2表达频率有高度显著性差异,成釉细胞瘤中阴性表达频率较高。
■在这些病变中已观察到干细胞标志物，表明肿瘤细胞获得了干细胞样的特性，这会影响患者的预后。具体来说,标记SOX2在牙源性囊肿和肿瘤中显示差异表达。SOX2在OKC中的高表达表明存在具有显著自我更新和增殖特性的干细胞,潜在的肿瘤行为。相比之下,成釉细胞瘤中SOX2的弱表达或缺失表明其肿瘤行为涉及不同的分子途径。
