PDF(Pubmed)
Abstract：
Grain size is an important appearance quality trait in rice, which also affects grain yield. In this study, a recombinant inbred line (RIL) population derived from a cross between indica variety 9311 and japonica variety Cypress was constructed. And 181 out of 600 RILs were sequenced, and a high-density genetic map containing 2842 bin markers was constructed, with a total map length of 1500.6 cM. A total of 10 quantitative trait loci (QTL) related to grain length (GL), grain width (GW), grain length-to-width ratio (LWR), and 1000-grain weight (TGW) were detected under two environments. The genetic effect of qGL4, a minor QTL for GL and TGW, was validated using three heterogeneous inbred family (HIF) segregation populations. It was further dissected into two closed linked QTL, qGL4.1 and qGL4.2. By progeny testing, qGL4.1 and qGL4.2 were successfully delimited to intervals of 1304-kb and 423-kb, respectively. Our results lay the foundation for the map-based cloning of qGL4.1 and qGL4.2 and provide new gene resources for the improvement of grain yield and quality in rice.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01447-y.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01447-y.
摘要：
粒级是水稻重要的外观品质性状,这也会影响谷物产量。在这项研究中,构建了一个重组自交系（RIL）种群，该种群来自in品种9311和粳稻品种Cypress之间的杂交。600个RIL中有181个被测序，并构建了包含2842个bin标记的高密度遗传图谱，地图总长度为1500.6cM。共有10个数量性状位点（QTL）与粒长（GL）有关，晶粒宽度(GW)，晶粒长宽比(LWR)，在两种环境下检测到1000粒重(TGW)。GL和TGW的次要QTLqGL4的遗传效应，使用三个异质近交系（HIF）分离种群进行了验证。它被进一步解剖成两个封闭连接的QTL，qGL4.1和qGL4.2.通过后代测试，qGL4.1和qGL4.2被成功地划分为1304kb和423kb的间隔，分别。本研究结果为qGL4.1和qGL4.2的图谱克隆奠定了基础,为提高水稻产量和品质提供了新的基因资源。
■在线版本包含补充材料，可在10.1007/s11032-024-01447-y获得。
■在线版本包含补充材料，可在10.1007/s11032-024-01447-y获得。
