Abstract：
This study focuses on the prevalence of Pseudomonas aeruginosa in various medical specimens. In addition, the investigates of this research shows the genetic analysis of pathogen-resistant isolates and chemical modifications to ciprofloxacin. A total of 225 specimens from men and women aged 30 to 60 were carefully collected and examined, including samples from wound, burn, urine, sputum, and ear samples. The data were obtained from AL Muthanna hospitals. PCR-RFLP and gene expression analysis were used to identify resistant strains and explore the genetic basis of antibiotic resistance. A ciprofloxacin derivative was synthesized and confirmed through FT-IR, 1H-NMR, and mass spectroscopy techniques then it was tested as antibacterial agent. Also, molecular docking study was conducted to predict the mechanism of action for the synthesized derivative. The results demonstrated that wound samples had the highest positive rate (33.7%) of P. aeruginosa isolates. The PCR-RFLP testing correlated ciprofloxacin resistance with gyrA gene mutation. Gene expression analysis revealed significant changes in the gyrA gene expression in comparison to the reference rpsL gene subsequent to exposure to the synthesized derivative. Furthermore, the molecular docking investigation illustrated the strategic positioning of the ciprofloxacin derivative within the DNA-binding site of the gyrA enzyme. The examination of genetic expression patterns manifested diverse effects attributed to the CIP derivative on P. aeruginosa, thus portraying it as a viable candidate in the quest for the development of novel antimicrobial agents. Ciprofloxacin derivative may offer new antimicrobial therapeutic options for treating Pseudomonas aeruginosa infections in wound specimens, addressing resistance and gyrA gene mutations.
摘要：
这项研究的重点是各种医学标本中铜绿假单胞菌的患病率。此外,这项研究的调查显示了病原体抗性分离株的遗传分析和环丙沙星的化学修饰。共搜集三十岁至六十岁男女共二百二十五份标本,并仔细检验,包括伤口样本,燃烧,尿液,痰,耳朵样本。数据来自ALMuthanna医院。采用PCR-RFLP和基因表达分析的方法鉴定耐药菌株,探索耐药的遗传基础。合成了环丙沙星衍生物，并通过FT-IR进行了确证，1H-NMR,和质谱技术，然后将其作为抗菌剂进行测试。此外，进行了分子对接研究，以预测合成衍生物的作用机理。结果表明，伤口样本中铜绿假单胞菌的阳性率最高（33.7％）。PCR-RFLP检测将环丙沙星耐药与gyrA基因突变相关。基因表达分析显示，与暴露于合成衍生物后的参考rpsL基因相比，gyrA基因表达发生了显着变化。此外，分子对接研究说明了环丙沙星衍生物在gyrA酶的DNA结合位点内的战略定位。遗传表达模式的检查显示了归因于CIP衍生物对铜绿假单胞菌的不同作用，因此将其描绘成寻求开发新型抗菌剂的可行候选者。环丙沙星衍生物可能为治疗伤口标本中的铜绿假单胞菌感染提供新的抗菌治疗选择。解决抗性和gyrA基因突变。
