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Abstract：
Accumulating evidence suggests that differentially expressed circular RNAs (circRNAs) play critical roles in immune cells of systemic lupus erythematosus (SLE) patients. Hsa_circ_0000479 has been studied in the field of cancer and infection, whereas seldom studied in autoimmune diseases. The aim of this study was to investigate the role and clinical value of neutrophil hsa_circ_0000479 in SLE.
The expression levels of hsa_circ_0000479 in both healthy individuals and SLE patients\' neutrophils were detected by qPCR and compared with those in peripheral blood mononuclear cells (PBMCs) . In addition, the correlation of hsa_circ_0000479 levels in neutrophils with the clinical and immunological features of SLE patients was also analysed.
The expression levels of hsa_circ_0000479 in the patients with SLE were significantly higher in neutrophils than that of PBMCs, and also significantly higher than that in healthy controls (HCs). Moreover, the expression levels of hsa_circ_0000479 in neutrophils were negatively associated with absolute neutrophil count and complement 3 (C3), whereas positively correlated with anti-dsDNA and anti-nucleosome antibodies in SLE. In addition, SLE patients with higher levels of hsa_circ_0000479 demonstrated more several clinical manifestations, including Raynaud\'s phenomenon, alopecia and leucopenia.
Hsa_circ_0000479 is up-regulated in neutrophils of SLE patients, and is also associated with several important laboratory indicators and clinical manifestations, suggesting that hsa_circ_0000479 in neutrophils was one of probable factors involved in the pathogenesis of SLE with potential clinical value.
Hsa_circ_0000479 was expressed in neutrophils and was considerably higher than that of PBMCs in SLE patients.The neutrophil hsa_circ_0000479 was correlated with laboratory parameters, including NEUT, C3, anti-dsDNA antibodies and AnuA, in addition to being associated with Raynaud’s phenomenon, alopecia, and leucopenia in patients with SLE.Hsa_circ_0000479 in neutrophils may play an influential role in SLE patients and will be important to understand the pathogenesis, stratification and treatment in SLE.
The expression levels of hsa_circ_0000479 in both healthy individuals and SLE patients\' neutrophils were detected by qPCR and compared with those in peripheral blood mononuclear cells (PBMCs) . In addition, the correlation of hsa_circ_0000479 levels in neutrophils with the clinical and immunological features of SLE patients was also analysed.
The expression levels of hsa_circ_0000479 in the patients with SLE were significantly higher in neutrophils than that of PBMCs, and also significantly higher than that in healthy controls (HCs). Moreover, the expression levels of hsa_circ_0000479 in neutrophils were negatively associated with absolute neutrophil count and complement 3 (C3), whereas positively correlated with anti-dsDNA and anti-nucleosome antibodies in SLE. In addition, SLE patients with higher levels of hsa_circ_0000479 demonstrated more several clinical manifestations, including Raynaud\'s phenomenon, alopecia and leucopenia.
Hsa_circ_0000479 is up-regulated in neutrophils of SLE patients, and is also associated with several important laboratory indicators and clinical manifestations, suggesting that hsa_circ_0000479 in neutrophils was one of probable factors involved in the pathogenesis of SLE with potential clinical value.
Hsa_circ_0000479 was expressed in neutrophils and was considerably higher than that of PBMCs in SLE patients.The neutrophil hsa_circ_0000479 was correlated with laboratory parameters, including NEUT, C3, anti-dsDNA antibodies and AnuA, in addition to being associated with Raynaud’s phenomenon, alopecia, and leucopenia in patients with SLE.Hsa_circ_0000479 in neutrophils may play an influential role in SLE patients and will be important to understand the pathogenesis, stratification and treatment in SLE.
摘要：
■越来越多的证据表明，差异表达的环状RNA（circRNAs）在系统性红斑狼疮（SLE）患者的免疫细胞中起关键作用。Hsa_circ_0000479已在癌症和感染领域进行了研究，而在自身免疫性疾病中很少研究。本研究的目的是探讨中性粒细胞hsa_circ_0000479在SLE中的作用和临床价值。
■通过qPCR检测健康个体和SLE患者中性粒细胞中hsa_circ_0000479的表达水平，并与外周血单核细胞（PBMC）中的表达水平进行比较。此外,还分析了中性粒细胞中hsa_circ_0000479水平与SLE患者临床和免疫学特征的相关性。
■SLE患者中性粒细胞中hsa_circ_0000479的表达水平明显高于PBMC,也显著高于健康对照组（HCs）。此外，中性粒细胞中hsa_circ_0000479的表达水平与中性粒细胞绝对计数和补体3（C3）呈负相关，而在SLE中与抗dsDNA和抗核小体抗体呈正相关。此外,hsa_circ_0000479水平较高的SLE患者表现出更多的临床表现，包括雷诺现象，脱发和白细胞减少症。
■Hsa_circ_0000479在SLE患者的中性粒细胞中上调，并且还与几个重要的实验室指标和临床表现有关，提示中性粒细胞中的hsa_circ_0000479是SLE发病的可能因素之一，具有潜在的临床价值。
Hsa_circ_0000479在SLE患者的中性粒细胞中表达，明显高于PBMC。中性粒细胞hsa_circ_0000479与实验室参数相关，包括NEUT,C3，抗dsDNA抗体和AnuA，除了与雷诺现象有关，脱发,SLE患者的白细胞减少。中性粒细胞中的Hsa_circ_0000479可能在SLE患者中起影响作用，对了解其发病机制具有重要意义。SLE的分层和治疗。
■通过qPCR检测健康个体和SLE患者中性粒细胞中hsa_circ_0000479的表达水平，并与外周血单核细胞（PBMC）中的表达水平进行比较。此外,还分析了中性粒细胞中hsa_circ_0000479水平与SLE患者临床和免疫学特征的相关性。
■SLE患者中性粒细胞中hsa_circ_0000479的表达水平明显高于PBMC,也显著高于健康对照组（HCs）。此外，中性粒细胞中hsa_circ_0000479的表达水平与中性粒细胞绝对计数和补体3（C3）呈负相关，而在SLE中与抗dsDNA和抗核小体抗体呈正相关。此外,hsa_circ_0000479水平较高的SLE患者表现出更多的临床表现，包括雷诺现象，脱发和白细胞减少症。
■Hsa_circ_0000479在SLE患者的中性粒细胞中上调，并且还与几个重要的实验室指标和临床表现有关，提示中性粒细胞中的hsa_circ_0000479是SLE发病的可能因素之一，具有潜在的临床价值。
Hsa_circ_0000479在SLE患者的中性粒细胞中表达，明显高于PBMC。中性粒细胞hsa_circ_0000479与实验室参数相关，包括NEUT,C3，抗dsDNA抗体和AnuA，除了与雷诺现象有关，脱发,SLE患者的白细胞减少。中性粒细胞中的Hsa_circ_0000479可能在SLE患者中起影响作用，对了解其发病机制具有重要意义。SLE的分层和治疗。
