PDF(Pubmed)
Abstract：
Broad-range 16S rRNA PCR and sequencing of 1,183 blood specimens from 853 unique patients yielded an interpretable sequence and bacterial identification in 29%, 16S rRNA amplification with uninterpretable sequences in 53%, and no amplification in 18%. This study highlights the potential utility of this technique in identifying fastidious gram-negative and anaerobic bacteria but the frequent recovery of environmental and contaminant organisms argues for its judicious use.
OBJECTIVE: The existing literature focuses on its performance compared to blood cultures in patients with sepsis, leaving a gap in the literature regarding other blood specimens in suspected infectious syndrome across the severity spectrum. We aimed to characterize its microbiological outcomes and provide insight into its potential clinical utility.
OBJECTIVE: The existing literature focuses on its performance compared to blood cultures in patients with sepsis, leaving a gap in the literature regarding other blood specimens in suspected infectious syndrome across the severity spectrum. We aimed to characterize its microbiological outcomes and provide insight into its potential clinical utility.
摘要：
广泛的16SrRNAPCR和来自853个独特患者的1,183个血液样本的测序产生了可解释的序列和细菌鉴定，占29％，16SrRNA扩增与不可解释的序列在53％，18%没有扩增。这项研究强调了该技术在鉴定挑剔的革兰氏阴性细菌和厌氧细菌方面的潜在用途，但环境和污染生物的频繁恢复证明了其明智的用途。重要的是现有的文献集中在与败血症患者的血培养相比，在严重程度范围内，有关可疑感染综合征的其他血液标本的文献中存在空白。我们旨在表征其微生物学结果，并提供其潜在临床应用的见解。
